华支睾吸虫排泄分泌抗原诱导RAW264.7细胞IL-33表达的研究

发布时间：2018-01-08 01:06

本文关键词：华支睾吸虫排泄分泌抗原诱导RAW264.7细胞IL-33表达的研究　出处：《中国病原生物学杂志》2016年10期 　论文类型：期刊论文

【摘要】：目的探讨华支睾吸虫排泄分泌抗原(ESA)对巨噬细胞系RAW264.7白介素33(IL-33)表达的影响,并探讨其机制。方法分别在ESA(60μg/ml)与RAW264.7细胞作用2、4、8、12h时检测IL-33 mRNA表达水平。用抗TNF-α中和抗体阻断TNF-α作用,通过RT-PCR及Western blot检测对ESA诱导RAW264.7细胞IL-33表达变化的影响。结果 ESA诱导RAW264.7细胞IL-33mRNA水平增高(P0.05);利用TNF-α中和抗体阻断TNF-α作用,可显著降低IL-33mRNA的含量(P0.05)。但ESA对IL-33蛋白水平的表达无显著影响(P0.05)。结论 ESA可诱导RAW264.7细胞IL-33mRNA高表达,该作用部分依赖于自分泌的炎性因子TNF-α作用。
[Abstract]:Objective to investigate the effect of excretory and secretory antigen of Clonorchis sinensis (ESA) on the expression of IL-33 in macrophage cell line RAW264.7. The mechanism was studied by using ESA(60 渭 g / ml and RAW264.7 cells respectively. The expression of IL-33 mRNA was detected at 12h, and the effect of TNF- 伪 was blocked by anti-TNF- 伪 neutralizing antibody. The effect of RT-PCR and Western blot on IL-33 expression in RAW264.7 cells induced by ESA was observed. ESA induced the increase of IL-33mRNA level in RAW264.7 cells (. P0.05; TNF- 伪 neutralizing antibody was used to block the effect of TNF- 伪. The content of IL-33mRNA was significantly decreased, but ESA had no significant effect on the expression of IL-33 protein. Conclusion ESA can induce high expression of IL-33mRNA in RAW264.7 cells. This effect is partly dependent on the autocrine inflammatory factor TNF- 伪.
【作者单位】：徐州医科大学生物化学与分子生物学研究中心江苏省脑病生物信息重点实验室;徐州医科大学感染与免疫实验室;
【基金】：国家自然科学基金项目(No.8157019,31302077) 2015年度江苏省高校优势学科建设工程项目(No.1506) 江苏省高校自然科学面上项目(No.16KJB310016)
【分类号】：R383.22
【正文快照】： ***IL-33是新近发现的IL-1家族成员,通过与细胞膜表面ST2受体(ST2L)结合,诱导Th2类细胞因子合成与释放,从而参与过敏性、感染性及纤维化等多种疾病的发生和发展[1]。在杜氏利什曼原虫、血吸虫、疟疾等寄生虫感染中,IL-33参与机体的抗感染免疫及免疫病理损伤[2],但其在华支睾吸

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