青春期雌性小鼠抑郁模型的建立及其意义

发布时间：2018-01-09 06:03

本文关键词：青春期雌性小鼠抑郁模型的建立及其意义　出处：《中华疾病控制杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的建立青春期雌性小鼠抑郁模型,为女性青春期抑郁症高发机制研究奠定基础。方法 C57BL/6J 18天雌性、雄性小鼠各16只分别随机分为雌性对照组和雌性组,雄性对照组和雄性组(n=8)。4组小鼠适应2d后于第20天测试行为学。雌性组和雄性组自第21天制备3周慢性轻度不可预知应激(chronic unpredictable mild stress,CUMS)模型,3周后再对4组进行行为学测试。结果造模前4组行为学均无统计学差异(均有P0.05)。造模后雌性组较雌性对照组体质量变化、糖水消耗百分比、旷场运动距离及中央区时间、高架十字迷宫开放臂时间减少(均有P0.05),悬尾和强迫游泳不动时间增加(均有P0.05);雄性组较雄性对照组体质量变化、糖水消耗百分比、旷场运动距离及中央区时间减少,强迫游泳不动时间增加(均有P0.05)。与造模前比较,雌性组糖水消耗百分比和中央区时间均减少(均有P0.05),悬尾和强迫游泳不动时间均增加(均有P0.05);雄性组较造模前糖水消耗百分比减少(t=6.02,P0.001),强迫游泳不动时间增加(t=2.91,P=0.013)。造模后雌性组较雄性组运动距离和开放臂时间均减少(均有P0.05),悬尾和强迫游泳不动时间均增加(均有P0.05)。结论成功造模后雌性组较雄性组抑郁样行为更加明显,表明青春期雌性小鼠比雄性小鼠对CUMS更加易感。
[Abstract]:Objective to establish the depression model of puberty female mice and to lay a foundation for the study of the high incidence of adolescent depression in female. Methods C57BL / 6J / 618 days female. 16 male mice were randomly divided into female control group and female group. The behavior of male control group and male group 8.4 mice were tested on the 20th day after adaptation for 2 days. The female group and male group prepared chronic mild unpredictable stress for 3 weeks from day 21 (P < 0.05). Chronic unpredictable mild stress. Results there was no significant difference in behavior in the four groups before the model was made (all P 0.05). The body weight of female group was different from that of female control group after the model was made. Percentage of consumption of sugar water, distance of open field movement and time of central area, time of open arm of elevated cross maze decreased (P 0.05), immobility time of suspended tail and forced swimming increased (P 0.05). Compared with the male control group, the body mass change, the percentage of sugar water consumption, the distance of open field movement and the time of central area decreased, and the immobility time of forced swimming increased in male group (all P 0.05). In female group, the percentage of sugar water consumption and the time of central area decreased (P 0.05), and the immobility time of tail suspension and forced swimming increased (P 0.05). The percentage of sugar water consumption in male group was decreased by 6.02g / P0.001g, and the time of forced swimming immobility was increased by 2.91. The movement distance and open arm time of female group were lower than that of male group (P0.05). The immobility time of tail suspension and forced swimming increased (P 0.05). Conclusion the depressive behavior of female group is more obvious than that of male group after successful modeling. The results showed that puberty female mice were more susceptible to CUMS than male mice.
【作者单位】：天津医科大学生物医学工程与技术学院神经生物学实验室;天津医科大学生物医学工程与技术学院;
【基金】：国家自然科学基金(81471393)
【分类号】：R749.4;R-332
【正文快照】： 抑郁症是以持续情绪低落为显著特征的全球性常见精神疾病,已成为世界排名第二自杀原因[1-2]。研究报道,青春期前儿童抑郁症无性别差异,进入青春期后女性抑郁发生率急剧增高,为男性青春期抑郁发病率的2~3倍,出现第一个发病高峰[3]。女性抑郁症的发病高峰为青春期、产后及更年期

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