双氧化酶2在5-羟色胺加重的小鼠结肠炎模型中的表达及作用

发布时间：2018-01-09 20:15

本文关键词：双氧化酶2在5-羟色胺加重的小鼠结肠炎模型中的表达及作用　出处：《安徽医科大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨5-羟色胺(5-HT)对双氧化酶2(Duox2)在小鼠结肠组织中的表达情况及其在炎症中的意义。方法选取6~8周龄的健康清洁级C57BL/6J(B6)雄性小鼠,随机分为5组(n=8):对照组自由饮用无菌蒸馏水;葡聚糖硫酸钠(DSS)(1.0%、2.5%)组自由饮用相应浓度的DSS溶液;5-HT组采用直肠灌药方式(1.0 mg/kg)给药,每3 d 1次;DSS+5-HT组同时给予1.0%DSS和5-HT。于造模第6天处死小鼠取结肠组织。通过结肠长度、疾病活动指数(DAI)评分和HE染色等方法评估肠道炎症程度,采用实时定量PCR(RT-PCR)检测小鼠结肠组织中白介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α和Duox2 mRNA表达水平,应用免疫组化检测结肠组织中Duox2蛋白的表达情况。结果对照组和5-HT组小鼠无肠炎表现;DSS(1.0%)组小鼠表现为轻度肠炎;DSS+5-HT组和DSS(2.5%)组有严重急性肠炎表现。IL-1β、IL-6和TNF-α等表达量随炎症程度加重而增加。Duox2 mRNA在5-HT组和DSS(1.0%)组表达增高(均P0.05),在DSS+5-HT和DSS(2.5%)组表达下调(均P0.05)。免疫组化结果显示Duox2蛋白主要表达于小鼠结肠上皮刷状缘及上皮细胞胞质中,与mRNA表达一致。结论 5-HT可能通过影响Duox2的表达在小鼠结肠炎的发生发展中发挥作用。
[Abstract]:Objective to study the effect of 5-hydroxytryptamine (5-HT) on dioxygenase 2 (Dox2). Methods healthy and clean C57BL / 6JJ B6 male mice were selected at the age of 6 weeks and 8 weeks old. They were randomly divided into 5 groups: the control group drank aseptic distilled water freely; Sodium dextran sulfate (DSS) and sodium dextran sulfate (DSS) group were free to drink the corresponding concentration of DSS solution. The 5-HT group was treated with rectal perfusion (1.0 mg / kg) once every 3 days. The DSS 5-HT group was given 1.0 DSS and 5-HT at the same time. On the 6th day, the mice were killed to take the colonic tissue and the colonic length was passed. Disease activity index (DAI) score and HE staining were used to evaluate the degree of intestinal inflammation, and real-time quantitative PCR- RT-PCR was used to detect interleukin-1 尾 in colonic tissue of mice. IL-6, TNF- 伪 and Duox2 mRNA expression levels. The expression of Duox2 protein in colonic tissue was detected by immunohistochemistry. Results No enteritis was found in mice of control group and 5-HT group. The mice in DSS1. 0) group showed mild enteritis; DSS 5-HT group and DSS group 2.5) have severe acute enteritis. IL-1 尾. The expression of IL-6 and TNF- 伪 increased with the severity of inflammation. The expression of. Duox2 mRNA was increased in 5-HT group and DSS 1. 0% group (all P 0.05). In the DSS 5-HT and DSS2. 5 groups, the expression was down-regulated (P0.05). Immunohistochemical results showed that Duox2 protein was mainly expressed in the brushes and cytoplasm of mouse colonic epithelium. Conclusion 5-HT may play a role in the pathogenesis and development of colitis by affecting the expression of Duox2.
【作者单位】：河南科技大学临床医学院河南科技大学第一附属医院消化内科;河南科技大学临床医学院河南科技大学第一附属医院检验科;河南科技大学临床医学院河南科技大学第一附属医院肝胆外科;首都医科大学附属北京康复医院消化内科;
【分类号】：R574.62
【正文快照】： IL-6、肿瘤坏死因子(TNF)-α和Duox2 mRNA表达水平,应用免疫组化检测结肠组织中Duox2蛋白的表达情况。结果对照组和5-HT组小鼠无肠炎表现;DSS(1.0%)组小鼠表现为轻度肠炎;DSS+5-HT组和DSS(2.5%)组有严重急性肠炎表现。IL-1β、IL-6和TNF-α等表达量随炎症程度加重而增加。Duox

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