四川凉山地区彝族家系KIR单体型分析

发布时间：2018-01-09 23:31

本文关键词：四川凉山地区彝族家系KIR单体型分析　出处：《免疫学杂志》2016年09期 　论文类型：期刊论文

【摘要】：目的了解四川凉山地区彝族家系KIR单体型的基因组成情况。方法采用多重PCR-SSP的方法对89个四川凉山地区彝族家系共364人的16个KIR基因位点进行基因分型;根据KIR基因型结合家系分析推导KIR单体型。对部分样本中某些不能确定拷贝数的KIR基因进行荧光定量PCR分析,再结合家系分析得到其KIR单体型。结果 89个四川凉山地区彝族家系中发现22种KIR基因型,21种单体型,其中3种A单体型,18种B单体型;并发现了2种较为独特的KIR单体型。结论四川彝族家系有其独特的KIR单体型种类,其基因组成具有以下规律:2DL1、2DP1总是和3DP1同时出现;有2DS1时则2DS4缺失,反之亦然;2DS2总是和2DL2同时出现。
[Abstract]:Objective to investigate the gene composition of KIR haplotype in Yi families in Liangshan area, Sichuan Province. Methods 16 KIR samples of 364 Yi families from 89 Yi families in Liangshan area, Sichuan Province were studied by using multiple PCR-SSP method. Gene loci were genotyped; The KIR haplotypes were deduced from KIR genotypic and pedigree analysis. Fluorescence quantitative PCR analysis was performed for some KIR genes in some samples which could not be determined copy number. The KIR haplotypes of 22 KIR genotypes and 18 B haplotypes were found in 89 Yi families in Liangshan, Sichuan province. Two unique KIR haplotypes were found. Conclusion there are unique KIR haplotypes in Sichuan Yi families, and their gene composition is as follows: 2DL1. 2DP1 always appeared at the same time as 3DP1; When there is 2DS 1, 2DS 4 is missing, and vice versa; The 2 DS 2 always appears at the same time as 2 D L 2.
【作者单位】：西南医科大学附属医院检验科;中国医学科学院输血研究所血液免疫室;
【分类号】：R392
【正文快照】： 杀伤细胞免疫球蛋白样受体(killer cellimmunoglobulin-like receptor,KIR)是NK细胞和某些T细胞表面表达的一组蛋白受体,它在NK细胞和T细胞免疫应答中起着重要作用。NK细胞可同时表达抑制性和激活性KIR受体分子,NK细胞发挥何种作用,主要取决于其表面抑制性和激活性KIR分子的综

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