补骨脂素对类风湿性关节炎小鼠模型的免疫调节作用

发布时间：2018-01-11 01:09

本文关键词：补骨脂素对类风湿性关节炎小鼠模型的免疫调节作用　出处：《中国实验动物学报》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨补骨脂素对II型胶原诱导的小鼠类风湿性关节炎的作用并初步探讨其作用的分子机制。方法选用DBA/1J小鼠,利用牛II型胶原诱导构建类风湿性关节炎小鼠模型。将造模成功的小鼠随机分为补骨脂素(PSO)、甲氨蝶呤(MTX)和模型对照(vehicle)三组,灌胃给药,观察关节改变情况并评分。称重并计算脾指数;流式细胞术测定脾淋巴细胞中Th1、Th2细胞数;ELISA检测血清相关炎症因子TNF-α、IL-6和IL-1β的表达量变化。结果与vehicle组相比,PSO组足踝肿胀、活动受限程度明显减轻;PSO组脾指数显著低于模型对照组,Th1淋巴细胞所占比例亦较Vehicle组显著下降,Th2淋巴细胞比例差异无显著性;ELISA结果显示PSO组血清TNF-α、IL-6和IL-1β显著低于Vehicle组。各项结果 PSO组与MTX组无统计学差别。结论补骨脂素通过调节Th1/Th2细胞平衡及抑制TNF-α、IL-6和IL-1β起到免疫调节作用,从而缓解RA进程。
[Abstract]:Objective to investigate the effect of psoralen on type II collagen-induced rheumatoid arthritis in mice and its molecular mechanism. Methods DBA/1J mice were selected. The mouse model of rheumatoid arthritis was induced by bovine type II collagen. The successful mice were randomly divided into psoralen (PSO). Methotrexate (MTX) group and model control group (control group) were administered intragastrically to observe the changes of joints and score, weigh and calculate spleen index. The number of Th1Th _ 2 cells in splenic lymphocytes was measured by flow cytometry. The expression of TNF- 伪 IL-6 and IL-1 尾 in serum was detected by ELISA. Results compared with vehicle group, the swelling of ankle and foot were observed. The degree of activity restriction was obviously reduced; The spleen index of PSO group was significantly lower than that of model control group. The percentage of Th1 lymphocyte in PSO group was significantly lower than that in Vehicle group. The results of ELISA showed serum TNF- 伪 in PSO group. IL-6 and IL-1 尾 were significantly lower than those in Vehicle group. There was no significant difference between PSO group and MTX group. Conclusion psoralen can regulate the balance of Th1/Th2 cells and inhibit TNF- 伪. IL-6 and IL-1 尾 play an immunomodulatory role, thus easing the RA process.
【作者单位】：山西医科大学实验动物中心实验动物与人类疾病动物模型山西省重点实验室;山西医科大学生物化学与分子生物学教研室;
【基金】：山西省实验动物专项资金项目(2009k03,2013k12) 山西省重点实验室建设项目(2015012001-09)
【分类号】：R285.5;R-332
【正文快照】： Corresponding author:CHEN Zhao-yang,E-mail:ccytycn@163.com类风湿性关节炎(rheumatoid arthritis,RA)是一种引发机体多个关节发生炎症、以关节疼痛、关节囊肿胀等病变为主要表现的全身性自身免疫性疾病。RA的发生发展与大量致炎细胞因子和炎症介质释放有关,最后引起关节部

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