巴贝西虫感染黑线仓鼠生物学特性的变化

发布时间：2018-01-11 19:42

本文关键词：巴贝西虫感染黑线仓鼠生物学特性的变化　出处：《中国比较医学杂志》2016年08期 　论文类型：期刊论文

【摘要】：目的建立巴贝西虫感染的黑线仓鼠模型,明确感染后黑线仓鼠生物学特性的变化规律,为巴贝西虫病的检测与防治提供基础数据资料。方法腹腔注射含巴贝西虫的血液感染黑线仓鼠,感染的第0、2、4、6、8、10、12、14、16、23、30、37天每次取5只动物采集抗凝血和全血,制备血涂片,通过吉姆萨染色检测虫体繁殖情况;分离血液总DNA,用REAL-TIME PCR检测巴贝西虫的在宿主体内的繁殖规律;用全自动生理生化检测仪测定血液生理生化指标;处死动物后分别采集心、肝、脾、肺、肾等器官,称重测定脏器系数;用ELISA法检测感染动物血清中IL-2浓度。结果感染后第4天黑线仓鼠体内的巴贝西虫数量最多,之后整体呈下降趋势,在第12天有一个短暂升高。感染动物的脏器系数变化最大的属于肝脏和脾脏,心、肺和肾脏系数在整个感染期内稍有波,均在正常值范围内。感染动物的血细胞均有波动,在第10、23天两次达峰值,其中白细胞变化最为剧烈;检测到变化的血液生化指标在第12天达峰值。感染动物血清中的IL-2在第10天达峰值,之后连续下降。结论感染巴贝西虫的黑线仓鼠具有典型的蜱传寄生虫病特点,病原侵入一周内达繁殖顶峰,且病原可在宿主体内长期潜伏。宿主免疫响应在第2周达到峰值,与免疫相关的脏器及血细胞有明显的应激反应。据此,可有针对性的开展巴贝西虫病的诊断与防治。
[Abstract]:Objective to establish a model of Cricetulus BABEI West infection after infection, clear Cricetulus biological characteristics, and provide basic information for the detection and prevention of BABEI West worm disease. Methods of intraperitoneal injection with BABEI West insect blood infection Cricetulus barabansis, each day 0,2,4,6,8,10,12,14,16,23,30,37 infection in 5 animal anti coagulation and blood collection preparation, blood smear, detection of parasite reproduction by Giemsa staining; separation of blood DNA, REAL-TIME PCR detection of BABEI west of worms in the propagation of the host; Determination of physiological and biochemical indexes of blood by automatic physiological and biochemical detection instrument; animal were sacrificed respectively after collecting heart, liver, spleen, lung, kidney and other organs weighing, determination of organ coefficient; ELISA method was used to detect the concentration of IL-2 infection in animal serum. Results fourth days after infection in the number of Cricetulus BABEI West worms, after a whole A downward trend, on the twelfth day there was a transient increase in infected animal organs. The maximum coefficient of variation belongs to the liver and spleen, heart, lung and kidney coefficient in the infection period a little wave, are within the normal range. All blood cell fluctuations of infected animal, in the peak two days 10,23. The most dramatic changes in white blood cells; blood biochemical index changes were detected in twelfth Tianda peak. Infection in animal serum IL-2 in the tenth Tianda peak, after a continuous decline. Conclusion the infection BABEI West insect Cricetulus with typical tick borne parasitic disease characteristics, pathogen invasion and reproduction within a week peak, long-term pathogen lurking in the host. The host immune response reached a peak in second weeks, and immune related organs and blood cell stress reaction obviously. Accordingly, there may be in order to carry out the diagnosis and treatment of echinococcosis of the west of BABEI.

【作者单位】：军事医学科学院实验动物中心;北京农业质量标准与检测技术研究中心;首都师范大学生物系;北京市兽药监察所;
【基金】：国家自然科学基金面上项目(31272385) “十二五”重大专项(2011ZXJ09201-031) 北京市自然科学基金面上项目(5152023) 病原微生物生物安全国家重点实验室开放课题(SKLPBS1435) 军事医学科学院创新基金(2012CXJJ022) 后勤“十二五”面上项目(CWS11J092)
【分类号】：R-332;R531
【正文快照】： babesiosis.Methods Healthy 5-week old Chinese hamsters were infected by intraperitoneal injection of blood containingBabesia.Blood samples were collected at 0,2,4,6,8,10,12,14,16,23,30,and 37 days after infection from 5hamsters at each time point.Blood s

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