富血小板血浆（PRP）对大鼠游离脂肪移植物存活作用的实验研究

发布时间：2018-01-12 13:08

本文关键词：富血小板血浆（PRP）对大鼠游离脂肪移植物存活作用的实验研究　出处：《华中科技大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的建立wistar大鼠游离脂肪移植的实验动物模型，观察富血小板血浆（PRP）对游离脂肪移植物存活的作用。方法实验采用自身对照，获取近交系wistar大鼠脂肪组织和PRP，于24只wistar大鼠背部分别注射以下三组脂肪移植物：A空白对照组（n=24），0.5ml脂肪组织+0.12ml生理盐水；B实验组1（PRP未激活组，n=24），0.5ml脂肪组织+0.1ml PRP+0.02ml生理盐水；C实验组2（PRP激活组，n=24），0.5ml脂肪组织+0.1ml PRP+0.02ml0.9%氯化钙。移植术后1月、2月、3月各处死8只大鼠，分别获取1月、2月和3月三组移植物标本，进行形态学和组织学观察，标本行HE染色，于光镜下观察脂肪组织存活情况，包括脂肪组织完整性、囊腔和空泡的形成、纤维化程度、炎症反应和血管密度，分别从以上方面进行病理学评分，采用配对t检验（SPSS13.0）对数据进行统计分析。结果PRP中血小板数（4871±635.5）明显高于全血中血小板数（617.6±61.30）（P＜0.0001）；形态学观察结果：移植后脂肪组织体积逐渐变小，质地变硬，周围形成一层包膜，表面可见血管网形成，术后1月，部分脂肪移植物已有明显吸收，，但无明显坏死、液化、脓肿形成等并发症发生，术后2月和3月时，已无法触及明显移植物，甚至完全消失不见；术后1月移植物体积比较，三组间均无明显差异（P＞0.05），而PRP激活组移植物直径明显大于空白组（P＜0.05）；组织学观察结果：术后1月，PRP激活组的血管密度明显高于空白对照组（P＜0.05）；术后3月，PRP激活组的炎症反应明显小于PRP未激活组和空白组（P＜0.05）；而其他病理观察指标：脂肪组织完整性、纤维化程度及囊腔空泡形成，在各时间段三组间均无统计学差异（P＞0.05）。结论在wistar大鼠模型上PRP对游离脂肪移植物的存活无明显促进作用。
[Abstract]:Objective to establish an experimental animal model of free fat transplantation in wistar rats and to observe the effect of platelet-rich plasma wistar on the survival of free fat grafts. Methods the adipose tissue and PRP of inbred wistar rats were obtained by self-control. The following three groups of fat grafts were injected into the back of 24 wistar rats. In the control group, 0. 12 ml of normal saline was injected into 0. 12 ml of adipose tissue. In group B, 0. 1 ml PRP 0. 02 ml normal saline was found in 0. 5 ml of adipose tissue. In group C, 0.1 ml PRP 0.02 ml 0.9% calcium chloride was found in 0. 5 ml adipose tissue in group 2 of PRP activation. January, February after transplantation. On March, 8 rats were killed and grafts were collected from January, January and March 3rd groups respectively. Morphological and histological observation was performed. The specimens were stained with HE, and the survival of adipose tissue was observed under light microscope. Including adipose tissue integrity, cystic and vacuolar formation, degree of fibrosis, inflammatory response and vascular density, pathological scores were performed from the above aspects. The data were statistically analyzed by paired t test (SPSS 13.0). Results the platelet count in PRP (4871 卤635.5) was significantly higher than that in whole blood (617.6 卤61.30) (P < 0.0001). Morphological observation: after transplantation, the volume of adipose tissue gradually became smaller, the texture became hard, a coating was formed around it, and the vascular network was formed on the surface. On January, some fat grafts had been absorbed obviously. However, there was no obvious necrosis, liquefaction, abscess and other complications. On February and March, the grafts could not be reached or even disappeared completely. On January, there was no significant difference in graft volume among the three groups (P > 0.05), but the diameter of graft in PRP activated group was significantly larger than that in blank group (P < 0.05). Results of histological observation: on January, the vascular density in PRP activated group was significantly higher than that in blank control group (P < 0.05). On March, the inflammatory response in PRP-activated group was significantly lower than that in non-activated PRP group and blank group (P < 0.05). Other pathological indicators: adipose tissue integrity, degree of fibrosis and cavitation formation were not significantly different among the three groups (P > 0.05). Conclusion PRP has no effect on the survival of free fat grafts in wistar rats.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R622.9

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