乳酸菌DM9054、DM9057的安全性评价

发布时间：2018-01-13 21:03

本文关键词：乳酸菌DM9054、DM9057的安全性评价　出处：《中国微生态学杂志》2011年09期 　论文类型：期刊论文

【摘要】：目的实验评价的2株乳酸菌DM9054和DM9057均分离于泡菜,分别具有降胆固醇和降血压功能,由于其功能性强,性状优良,故本实验对其安全性进行评价,为应用于食品药品生产提供参考依据。方法通过急性毒性试验、耐药性、机体指标、有害代谢产物的评价试验以及其他安全性评价试验检测两实验菌株的安全性。结果在一次性分别使用DM9054和DM9057大剂量灌胃后,动物体征指标无异常;两菌株对部分抗生素具有抗性,但不携带可转移的耐药质粒;使用实验菌株后,机体指标MDA、GSH和SAA的变化基本正常;菌株代谢不产生胆盐羟化酶、硝基还原酶和氨基脱羧酶,但产生乳酸;无溶血作用,长期使用,不发生易位现象。结论乳酸菌DM9054和DM9057总体上是安全的。
[Abstract]:Objective two strains of lactic acid bacteria, DM9054 and DM9057, were isolated from kimchi and had the function of lowering cholesterol and blood pressure respectively. Therefore, this study evaluated its safety and provided reference for its application in food and drug production. Methods through acute toxicity test, drug resistance, body indicators. The safety of the two experimental strains was tested by the evaluation test of harmful metabolites and other safety evaluation tests. Results after a single oral administration of DM9054 and DM9057 respectively. No abnormal animal signs were found. The two strains were resistant to some antibiotics, but did not carry transferable resistant plasmids. The changes of MDA-GSH and SAA were basically normal after using the experimental strains. The strain metabolism did not produce bile salt hydroxylase, nitroreductase and amino decarboxylase, but produced lactic acid. No hemolysis, long term use and no translocation. Conclusion Lactic acid bacteria DM9054 and DM9057 are safe on the whole.
【作者单位】：大连医科大学微生态学教研室;
【基金】：国家“863”计划(2007A10Z356) 国家“973”计划(2007CB513006)
【分类号】：TS201.3
【正文快照】： 由于适量食用益生菌对身体有益[1],故乳酸菌被广泛应用于乳制品发酵、功能性食品配料、食品添加剂、膳食补充剂、饲料微生态制剂的生产中,以达到调节肠道菌群的目的。随之而来的是乳酸菌的安全性也受到了人们的广泛关注。尽管我国没有制定乳酸菌安全性评价的相关准则,但检测

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