白念珠菌肌醇多磷酸激酶Kcs1的功能

发布时间：2018-01-14 06:03

本文关键词：白念珠菌肌醇多磷酸激酶Kcs1的功能　出处：《微生物学通报》2017年10期 　论文类型：期刊论文

【摘要】：【目的】鉴定白念珠菌肌醇多磷酸激酶Kcs1蛋白,并探索Kcs1在该病原菌细胞自噬、菌丝发育及致病过程中的功能。【方法】采用二步PCR介导的同源重组方法,构建白念珠菌KCS1基因缺失菌株kcs1Δ/Δ及回补菌株KCS1c;采用氮饥饿敏感性测定及GFP-Atg8自噬报告系统,测定KCS1缺失对白念珠菌自噬过程的影响;采用菌丝诱导培养,测定KCS1缺失对白念珠菌菌丝发育能力的影响;采用巨噬细胞模型及小鼠系统性感染模型,分析KCS1缺失对白念珠菌感染宿主能力的影响。【结果】KCS1缺失造成白念珠菌氮饥饿耐受能力降低,氮饥饿条件下自噬相关蛋白Atg8的降解及转运水平下降,菌丝发育变缓,对巨噬细胞耐受及损伤能力减弱,但不影响菌株的小鼠系统性感染能力。【结论】白念珠菌肌醇多磷酸激酶Kcs1在细胞自噬、菌丝发育、与巨噬细胞相互作用等方面发挥重要作用。
[Abstract]:[objective] to identify the inositol polyphosphokinase (Kcs1) protein of Candida albicans and to explore the role of Kcs1 in autophagy of the pathogen. [methods] A two-step PCR mediated homologous recombination method was used to construct Candida albicans KCS1 gene deletion strain kcs1 螖 / 螖 and complementary strain KCS1c. The effects of KCS1 deficiency on the autophagy process of Candida albicans were determined by nitrogen starvation sensitivity test and GFP-Atg8 autophagy report system. The effect of KCS1 deficiency on the development ability of Candida albicans was determined by hyphal induction culture. The effects of KCS1 deletion on the host ability of Candida albicans infection were analyzed by using macrophage model and systemic infection model in mice. [results] the ability of nitrogen starvation tolerance of Candida albicans was reduced by KCS1 deficiency. Under the condition of nitrogen starvation, the degradation and transport of autophagy associated protein Atg8 decreased, the mycelium development slowed down, and the ability of tolerance and damage to macrophages decreased. [conclusion] the inositol polyphosphokinase Kcs1 of Candida albicans plays an important role in autophagy, mycelium development and interaction with macrophages.
【作者单位】：南开大学微生物学系分子微生物学与技术教育部重点实验室;
【基金】：国家自然科学基金项目(No.31670146,81471923,31400132) 天津市自然科学基金项目(No.17JCZDJC33300,15JCQNJC09300)~~
【分类号】：R379.4
【正文快照】： 白念珠菌(Candida albicans)是临床常见的条件致病真菌之一,有81个亚种,其中11个亚种对人体存在致病性[1]。该真菌在正常情况下以共生形式广泛存在于人体口腔、肠道、呼吸道及阴道之中,不会引起疾病。但近年来随着大剂量抗生素、激素、免疫抑制剂的使用,以及临床大型手术病例

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