裸鼠人来源性子宫腺肌病造模方法的比较

发布时间：2018-01-14 07:15

本文关键词：裸鼠人来源性子宫腺肌病造模方法的比较　出处：《中国实验动物学报》2017年01期 　论文类型：期刊论文

【摘要】：目的通过不同的造模方法建立裸鼠人来源性子宫腺肌病模型,筛选出理想的造模方法,为研究子宫腺肌病发病机制及相关治疗方案提供理想的动物模型。方法将80只雌性裸鼠随机分为腹腔种植组、皮下种植组、腹腔注射组和皮下注射组,每组20只。各模型组分别采用不同造模方法建立裸鼠人来源性子宫腺肌病模型,术后4周观察移植物的体积及组织形态学。结果腹腔种植组建模成功率95%,皮下种植组建模成功率45%,腹腔注射法及皮下注射法成模率0%。结论腹腔种植法建立子宫腺肌病裸鼠模型成模率高,稳定性良好,是一种理想的人来源性子宫腺肌病动物模型。
[Abstract]:Objective through different modeling methods to establish the nude mouse model of adenomyosis of human origin, selected the ideal modeling method, to provide an ideal animal model for studying the pathogenesis of adenomyosis and related treatment. Methods 80 female mice were randomly divided into intraperitoneal injection group, subcutaneous implant group, intraperitoneal injection group and subcutaneous injection group, 20 rats in each group. The model group respectively using different modeling methods to establish the origins of the nude mice model of adenomyosis, 4 weeks after operation to observe displacement volume and histomorphology of plants. Results intraperitoneal injection group modeling success rate was 95%, the rate of successful model 45% planted under the skin, subcutaneous injection and intraperitoneal injection model establishment of adenomyosis in nude mice model was high, the rate of 0%. conclusion peritoneal method has good stability, is an ideal source of human adenomyosis animal model.

【作者单位】：广州中医药大学;广州中医药大学第一附属医院;
【基金】：国家自然科学基金项目(NO 81473715)
【分类号】：R711.71;R-332
【正文快照】： 子宫腺肌病近年来其发病率呈不断上升趋势,已成为妇科常见疑难病[1]。渐进性痛经、不孕为子宫腺肌病最主要的临床表现,痛经发生率高达77.8%[2],严重影响患者生活和工作。多年来,对于子宫腺肌病生理病理机制以及治疗效果的探索仍存在诸多空白,但因在人体中很难进行对照研究,限

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