中药栀子提取物GJ-4对亚急性衰老致痴呆小鼠学习记忆的保护作用

发布时间：2018-01-15 07:43

本文关键词：中药栀子提取物GJ-4对亚急性衰老致痴呆小鼠学习记忆的保护作用　出处：《中国新药杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:GJ-4是从中药栀子中提取分离出的一种藏红花色素富集物,本研究采用D-半乳糖联合亚硝酸钠所致亚急性衰老痴呆小鼠模型来评价GJ-4的学习记忆保护作用。方法:腹腔注射D-半乳糖(120mg·kg~(-1))和亚硝酸钠(90 mg·kg~(-1))诱导小鼠痴呆模型,同时各组小鼠分别灌胃(ig)给予GJ-4或阳性对照多奈哌齐以及脑复康,连续8周。采用跳台实验和Morris水迷宫检测小鼠行为学能力,并检测脑组织和血清中乙酰胆碱酯酶(Ach E)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)等相关指标,HE染色光镜下观察小鼠海马CA1区神经元变化。结果:与模型组比较,GJ-4组能明显缩短小鼠跳台潜伏期和减少跳台错误次数,延长小鼠在Morris水迷宫目标象限的游泳时间和增加穿越目标平台次数,表明GJ-4可提高亚急性衰老小鼠的学习记忆力和空间辨识能力。此外,GJ-4可降低脑组织中Ach E活性,保护海马CA1区神经元,并可不同程度提高血清及脑组织中SOD活性和GSH含量,降低MDA含量。结论:GJ-4对亚急性衰老痴呆小鼠的学习记忆能力有一定的保护作用,其作用机制可能与抑制Ach E和减少氧化应激损伤有关。
[Abstract]:Objective to study the extraction and separation of saffron pigment from Gardenia jasminoides L. In this study, the model of subacute senile dementia induced by D-galactose combined with sodium nitrite was used to evaluate the protective effect of GJ-4 on learning and memory. Methods: 120 mg 路kg ~ (-1) of Dgalactose (Dgalactose) was injected intraperitoneally. )) and sodium nitrite (90 mg 路kg ~ (-1))) were used to induce dementia model in mice. At the same time each group of mice were given GJ-4 or positive control Donepezil and Naofukang for 8 weeks respectively. The behavioral ability of mice was tested by platform jumping test and Morris water maze. The levels of acetylcholinesterase (Ach), malondialdehyde (MDAA), superoxide dismutase (SOD) and glutathione (GSH) in brain tissue and serum were determined. Results: compared with the model group, GJ-4 group could significantly shorten the latency of platform jumping and reduce the number of platform errors. Prolonging the swimming time of mice in the target quadrant of Morris water maze and increasing the number of crossing target platform showed that GJ-4 could improve the learning memory and spatial identification ability of subacute aging mice. GJ-4 can decrease the activity of Ach E in brain tissue, protect the neurons in CA1 area of hippocampus, and increase the activity of SOD and the content of GSH in serum and brain tissue to some extent. Conclusion: GJ-4 has a protective effect on learning and memory ability of subacute senile dementia mice, and its mechanism may be related to the inhibition of Ach E and the reduction of oxidative stress injury.
【作者单位】：暨南大学;中国医学科学院药物研究所天然药物活性物质与功能国家重点实验室;
【基金】：国家自然科学基金重点项目(81630097)
【分类号】：R285.5;R-332
【正文快照】： 与功能国家重点实验室,北京100050)随着我国人口老龄化进程的日益加剧,衰老及年龄相关疾病已成为当前日益严重的社会和医学问题[1]。其中老年痴呆尤为突出,它是一种以进行性痴呆为主要特征的神经退行性疾病,其临床主要表现为记忆力衰退和认知功能障碍,在中国老年性痴呆主要高

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