卵清蛋白、金黄色葡萄球菌肠毒素B和维生素D类似物诱导小鼠特应性皮炎模型（英文）

发布时间：2018-01-15 11:45

本文关键词：卵清蛋白、金黄色葡萄球菌肠毒素B和维生素D类似物诱导小鼠特应性皮炎模型（英文）　出处：《中南大学学报(医学版)》2017年09期 　论文类型：期刊论文

【摘要】：目的:阐明利用卵清蛋白(ovalbumin,OVA)、金黄色葡萄球菌肠毒素B(staphylococcal enterotoxin B,SEB)和维生素D类似物(calcipotriene ointment,CO)局部刺激小鼠背部建立特应性皮炎(atopic dermatitis,AD)动物模型的方法。方法:将BALB/c小鼠背部去毛随机分为3组:对照组,OVA/SEB,OVA/SEB/CO,分别给予相应的处理。隔天给药并拍照记录皮损状态,于第15天处死小鼠,处死前用皮肤共聚焦显微镜测量表皮厚度。用ELISA方法检测血清IL-2,IL-4,IL-31,干扰素(interferon,IFN)-γ,肿瘤坏死因子(tumor necrosis factor,TNF)-α,神经生长因子(nerve growth factor,NGF)和IgE水平,HE染色检测皮损处炎症情况,实时荧光定量PCR检测皮损处上述因子的mRNA表达水平。结果:OVA/SEB/CO组皮损处炎症细胞浸润和表皮增厚明显,血清IgE水平显著增高,血清和皮损处IL-4,IL-31,IFN-γ,TNF-α和NGF表达水平均显著高于对照组或OVA/SEB组。结论:OVA/SEB/CO局部刺激可短时间、低成本地诱导BALB/c小鼠皮肤AD样改变。
[Abstract]:Objective : To clarify the method of establishing animal model of atopic dermatitis ( AD ) on the back of BALB / c mice by local stimulation of egg white protein ( OVA ) , Staphylococcus aureus Enterotoxin B ( LPS ) and vitamin D analog ( CO ) . Methods : The levels of IL - 2 , IL - 4 , IL - 31 , interferon ( IFN ) - 纬 , tumor necrosis factor ( TNF ) - 伪 , nerve growth factor ( NGF ) and IgE were measured by ELISA .

【作者单位】：中南大学湘雅三医院皮肤科;中南大学基础医学院生理学系;中南大学湘雅二医院皮肤科;
【基金】：supported by the Natural Science Foundation of Hunan Province(2015JJ6120) the Health and Family Planning Research Foundation of Hunan Province(B2015-34) Development and Reform Commission Project of Hunan Province([2014]658),China
【分类号】：R-332;R758.2
【正文快照】： Atopic dermatitis(AD)is one of the most commonchronic skin inflammatory diseases that affect 10% 20%ofinfants and 1% 3%of adults worldwide.The main featureof AD is the presence of eczematoid,dry and pruritic skinlesions[1-2].The pathogenesis of AD is m

【相似文献】