高脂饮食对NAFLD模型大鼠肠道菌群及血清LPS水平的影响

发布时间：2018-01-16 15:28

本文关键词：高脂饮食对NAFLD模型大鼠肠道菌群及血清LPS水平的影响　出处：《安徽医科大学学报》2017年09期 　论文类型：期刊论文

【摘要】：目的观察高脂饮食诱导的非酒精性脂肪肝病(NAFLD)模型大鼠肠道菌群及血清内毒素(LPS)水平的变化,探讨肠道菌群改变及LPS血症在NAFLD发病过程中的作用。方法雄性SD大鼠12只随机分为对照组和高脂组,对照组以普通饮食饲养,高脂组给予高脂饲料喂养,于实验第16周末处死大鼠,采用全自动生化分析仪测定血清生化指标,电镜观察两组大鼠肝脏病理改变,ELISA法检测血清LPS、白介素(IL)-1β、IL-18水平的变化,通过实时荧光定量PCR法检测粪便大肠杆菌和乳酸杆菌的表达水平。结果高脂组大鼠血清谷丙转氨酶、谷草转氨酶、三酰甘油、总胆固醇、极低密度脂蛋白水平较对照组明显升高,高密度脂蛋白水平明显降低,差异均有统计学意义(P0.05);与对照组相比,高脂组大鼠血清LPS、IL-1β、IL-18水平明显升高,差异均有统计学意义(P0.05)。与对照组相比,高脂组大鼠的的大肠杆菌显著升高,而乳酸杆菌明显减少,差异有统计学意义(P0.05)。血清LPS水平与大肠杆菌量存在相关性。结论高脂饮食诱导的NAFLD模型大鼠存在LPS血症和肠道菌群的改变,不合理的饮食结构及粪便菌群的改变在NAFLD的发病过程中起着重要的作用。
[Abstract]:Objective to observe the changes of intestinal flora and serum endotoxin level in NAFLD- induced non-alcoholic fatty liver disease rats induced by high fat diet. Methods Twelve male Sprague-Dawley rats were randomly divided into control group and hyperlipidemia group. The rats in the high-fat group were fed with high-fat diet. The rats were killed at the 16th week of the experiment. The serum biochemical indexes were measured by automatic biochemical analyzer and the pathological changes of liver were observed by electron microscope. The changes of serum LPSand IL-18 levels were detected by ELISA. Results Serum alanine aminotransferase, alanine aminotransferase, triacylglycerol and total cholesterol were detected by real-time fluorescence quantitative PCR. The level of very low density lipoprotein was significantly higher than that of the control group, and the level of high density lipoprotein was significantly lower than that of the control group (P 0.05). Compared with the control group, the serum levels of IL-1 尾 -IL-18 in the hyperlipidemia group were significantly higher than those in the control group (P 0.05). The Escherichia coli level in the high-fat group was significantly higher than that in the control group, while the number of Lactobacillus was significantly decreased. The difference was statistically significant (P 0.05). The level of serum LPS was correlated with the amount of Escherichia coli. Conclusion the NAFLD model rats induced by high fat diet have LPS and intestinal flora changes. Unreasonable diet and fecal microflora play an important role in the pathogenesis of NAFLD.
【作者单位】：安徽医科大学第一附属医院临床营养科;
【基金】：安徽省年度重点科研计划项目(编号:1604a0802081)
【分类号】：R-332;R575
【正文快照】： 2017-05-09接收非酒精性脂肪肝(nonalcoholic fatty liver dis-ease,NAFLD)是指除外酒精和其他明确肝脏损伤因素所致的,以弥漫性肝细胞大泡性脂肪变性为主要特征的临床病理综合征[1]。至今关于NAFLD的发病机制的研究尚未完全统一,越来越多的研究[2]报道菌群紊乱、细菌移位及肠

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