百合疏肝安神汤对焦虑性抑郁症模型大鼠细胞因子及单胺类神经递质表达的影响

发布时间：2018-01-16 17:03

本文关键词：百合疏肝安神汤对焦虑性抑郁症模型大鼠细胞因子及单胺类神经递质表达的影响　出处：《中国临床药理学杂志》2017年21期 　论文类型：期刊论文

【摘要】：目的研究百合疏肝安神汤对焦虑性抑郁症模型大鼠细胞因子及单胺类神经递质表达的影响。方法 SD大鼠按体重被随机分为7组:模型组、阳性药对照组(文拉法辛6.75 mg·kg~(-1))、高中低3个剂量实验组(百合疏肝安神汤,24.28,12.24,6.12 g·kg~(-1)),空白对照组(正常饲养)和溶媒组(皮下注射0.9%NaCl+5%二甲基亚砜)。经连续21 d慢性束缚应激+孤笼饲养,并皮下注射皮质酮(30 mg·kg~(-1))来复制焦虑性抑郁症动物模型。造模同时,灌胃给药。用酶联免疫吸附法检测血浆、前额叶皮层(PFC)、海马和杏仁核组织中肿瘤坏死因子-α(TNF-α)含量;以免疫印迹法检测PFC、海马和杏仁核组织中吲哚胺2,3-双加氧酶(IDO)表达;用HPLC-ECD法测定前额叶皮层、海马和杏仁核中单胺类神经递质5-羟色胺(5-HT)、多巴胺(DA)和去甲肾上腺素(NE)的含量。结果血浆TNF-α含量、PFC中IDO的OD值、PFC中5-HT含量、海马中DA含量、杏仁核中NE含量:空白对照组与模型组分别(53.00±7.36),(96.75±10.10)pg·mL~(-1);0.40±0.06,0.79±0.08;(477.10±63.56),(231.94±31.51)ng·mg~(-1);(432.08±30.00),(249.41±25.35)ng·mg~(-1);(829.55±116.67)ng·mg~(-1),(461.49±70.00)ng·mg~(-1),与空白对照组比较,模型组差异均有统计学意义(P0.05或P0.01)。血浆TNF-α含量、PFC的IDO的OD值、PFC的5-HT含量、海马DA含量、杏仁核NE含量:高剂量实验组分别为(73.62±7.46)pg·mL~(-1)与(0.55±0.08)及(362.60±42.53),(328.85±30.22),(664.40±65.05)ng·mg~(-1),与模型组比较差异均有统计学意义(P0.05或P0.01)。结论百合疏肝安神汤可能是通过调节大鼠血浆和大脑组织中细胞因子和单胺类神经递质,起到抗焦虑抑郁的作用。
[Abstract]:Objective to study the effect of lily Shugan Anshen decoction on the expression of cytokines and monoamine neurotransmitters in rats with anxiety depression. Methods Sprague-Dawley rats were randomly divided into 7 groups according to their body weight: model group. The positive drug control group (venlafaxine 6.75 mg 路kg ~ (-1)) and the middle and low dose three experimental groups (lily Shugan Anshen decoction 24.28 ~ 12.24). 6.12 g 路kg ~ (-1). The blank control group (normal feeding) and the solvent group (0.9 NaCl 5% dimethyl sulfoxide were injected subcutaneously) were fed in solitary cage under chronic restraint stress for 21 days. The animal model of anxiety depression was established by subcutaneous injection of corticosterone (30 mg 路kg ~ (-1)). At the same time, the animal model was administered by intragastric administration. Plasma was detected by enzyme-linked immunosorbent assay (Elisa). The content of tumor necrosis factor- 伪 (TNF- 伪) in the prefrontal cortex, hippocampus and amygdala; The expression of indomethylamine 3-dioxygenase (IDO) in PFC, hippocampus and amygdala was detected by Western blot. The monoamine neurotransmitters 5-HT in prefrontal cortex, hippocampus and amygdala were determined by HPLC-ECD method. Results the contents of plasma TNF- 伪 and 5-HT in IDO and DA in hippocampus were determined by the contents of dopamine (DA) and norepinephrine (NE). The content of NE in amygdala: the blank control group and the model group were 53.00 卤7.36, respectively. 0.40 卤0.06U 0.79 卤0.08; 477.10 卤63.56ng 路mg ~ (-1) (231.94 卤31.51ng 路mg ~ (-1)); (2) 432.08 卤30.00GG ~ (-1) (249.41 卤25.35) ng 路mg ~ (-1); 829.55 卤116.67 ng 路mg ~ (-1) (P < 0.01), 461.49 卤70.00ng 路mg ~ (-1) (P < 0.01), compared with the control group. There were significant differences in the model group (P0.05 or P0.01). The plasma TNF- 伪 content, the OD value of IDO, the 5-HT content of PFC and the content of DA in hippocampus. The contents of NE in the amygdala were 73.62 卤7.46 PG 路mL ~ (-1), 0.55 卤0.08) and 362.60 卤42.53, respectively. 664. 40 卤65.05 ng 路mg ~ (-1)). Compared with the model group, the differences were statistically significant (P0.05) or P0.01.ConclusionLiliang Shugan Anshen decoction may regulate cytokines and monoamine neurotransmitters in plasma and brain tissue of rats. Play the role of anti-anxiety and depression.
【作者单位】：湖南中医药大学湖南省中药粉体与创新药物省部共建国家重点实验室培育基地;湖南中医药大学第一附属医院;
【基金】：国家自然科学基金资助项目(81403387) 湖南省科技厅重点基金资助项目(2015DK3003)
【分类号】：R285.5;R-332
【正文快照】： 杨蕙2,孟盼1(1.湖南中医药大学湖南省中药粉体与创新药物省部共建国家重点实验室培育基地,长沙410208;2.湖南中医药大学第一附属医院,长沙410007)DU Qing1,YANG Qin2,ZHAO Hong-qing1,WANG Yu-hong1,2,LING Jia1,HAN Yuan-shan2,YANG Hui2,MENG Pan1(1.Hunan Key Laboratory o

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