620nm低能量红光对骨髓间充质干细胞增殖的影响

发布时间：2018-01-17 13:02

  本文关键词：620nm低能量红光对骨髓间充质干细胞增殖的影响　出处：《华中科技大学》2012年硕士论文　论文类型：学位论文

  更多相关文章： 骨髓间充质干细胞 增殖 光生物调节作用

【摘要】：目的探讨发光二极管（Light-emitting Liode,LED）发射的波长620nm的低能量红光对SD大鼠骨髓间充质干细胞（Bone Mesenchymal Stem Cells,BMSCs）增殖的影响。 方法采用密度梯度离心法分离SD大鼠BMSCs，再经贴壁法筛选，取生长良好,呈对数生长期的第3代BMSCs，波长620nm的LEDs置于细胞上方2cm处，测得功率密度为6.67mW/cm2。根据不同照射时间所对应的能量密度，将BMSCs分别设置0、0.5、1和2J/cm2组，其中0J/cm2组为对照组，其他组为实验组,红光每天照射1次。采用细胞计数试剂盒-8(CCK-8)检测各组细胞红光照射前3天的细胞增殖活性，EdU（5-Ethynyl-2-deoxyundine）细胞增殖检测试剂盒和流式细胞仪分别检测各组细胞红光照射第3天后，DNA复制活性和细胞周期变化。 结果BMSCs经620nm低能量红光照射第3天后，实验组细胞与对照组的相比，增殖活性和DNA复制活性显著增强，(S、G2-M)期细胞数量显著增多，差异具有统计学意义（p0.05），其中0.5J/cm2组效果最显著。 结论LED发射的620nm低能量红光照射SD大鼠BMSCs第3天后，可有效促进其体外增殖。
[Abstract]:Objective to study the Light-fitting Liode of light emitting diodes (LEDs). The bone Mesenchymal Stem Cells of bone marrow mesenchymal stem cells (BMSCs) of SD rats was induced by low-energy red light at 620nm. Effects of BMSCs) on proliferation. Methods BMSCs were isolated from SD rats by density gradient centrifugation. The third generation BMSCs with good growth and logarithmic growth was selected by adherent method. The wavelength of 620nm LEDs was placed at 2 cm above the cell, and the power density was 6.67 MW / cm ~ (2). According to the energy density corresponding to different irradiation time, the BMSCs was set at 0. The 0. 5 J / cm2 and 2 J / cm2 groups were the control group and the other groups were the experimental group. The cell proliferation activity of each group was detected 3 days before red light irradiation by cell counting kit -8 CCK-8. The proliferation test kit and flow cytometry were used to detect the red light of each group after 3 days of irradiation. DNA replication activity and cell cycle changes. Results after BMSCs was irradiated with 620nm low energy red light for 3 days, the proliferative activity and DNA replication activity of the experimental group were significantly increased compared with those of the control group. The number of G2-Mphase cells increased significantly, and the difference was statistically significant (P 0.05). The effect of 0.5 J / cm 2 group was the most significant. Conclusion LED emission of 620nm low energy red light irradiation on the third day of BMSCs in SD rats can effectively promote the proliferation of SD rats in vitro.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R329

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