玉米须提取物对糖尿病足小鼠模型血糖、血脂及血液流变学的影响

发布时间：2018-01-17 17:19

本文关键词：玉米须提取物对糖尿病足小鼠模型血糖、血脂及血液流变学的影响　出处：《中医药学报》2016年04期 　论文类型：期刊论文

【摘要】：目的:探讨玉米须提取物对链脲佐菌素(STZ)诱导的糖尿病足模型血糖、血脂及血液流变学的影响。方法:昆明种小鼠150只,适应性饲养1周,随机将150只小鼠分成5组,30只/组,分别为空白组、模型对照组、玉米须提取液高剂量组、中剂量组、低剂量组。空白组喂以标准饲料,除空白组外其余组喂以高脂高糖饮食至实验结束。11周后除空白组外均造模,造模前禁食12 h,自由饮水,按每天40mg/kg剂量,将STZ用浓度为0.1 mmol/L柠檬酸缓冲液(p H4.2)溶解,给小鼠做腹腔注射1次/d,连续5天。除空白组和模型对照组外,其余3组玉米须提取物按照400、200、100 mg/kg,配成浓度为20、10、5g/L,灌胃体积均为0.2 m L/10 g灌胃给药。给药第30天,给药后1 h,禁食12 h,小鼠尾部取血,观察小鼠用药前后的血糖、血脂及血液流变学的指标变化。结果:给药后玉米须提取物高、中、低剂量组与模型对照组相比较均具有降低糖尿病足小鼠模型血糖的作用P0.01,且呈现剂量依赖性,组间有差异P0.05,高剂量组要明显优于低剂量组的降糖效果;给药后高、中、低剂量组与模型对照组相比较均具有改善血脂、血液流变学的作用P0.05,且呈现剂量依赖性,组间有差异P0.05,高剂量组要明显优于低剂量组改善血脂、血液流变学的作用。结论:玉米须提取物能够有效改善小鼠糖尿病足模型血糖、血脂、血液流变学的作用,且改善的效果随着剂量浓度的增高而增加。
[Abstract]:Objective: to investigate the effects of Corn whisker extract on blood glucose, blood lipid and hemorheology of diabetic foot model induced by streptozotocin (STZ). Methods: 150 Kunming mice were fed adaptively for one week. 150 mice were randomly divided into 5 groups (n = 30): blank group, model control group, high dose group, middle dose group and low dose group. The rest groups except the blank group were fed with high fat and high sugar diet until the end of the experiment. After 11 weeks, all the models were made except the blank group, fasting for 12 hours before making the model, drinking freely, at the dose of 40mg / kg per day. STZ was dissolved with 0.1 mmol/L citric acid buffer solution (pH4.2). Mice were injected intraperitoneally for 5 days, except blank group and model control group. The other three groups were treated with 400,200,100 mg / kg corn hair extract with a concentration of 20 ~ 10g / L ~ (-1) ~ (-1) ~ (-1) ~ (-1) 路kg ~ (-1). The volume of gavage was 0.2 mL / 10 g by gavage. On the 30th day, 1 hour after administration, 12 hours after fasting, blood was taken from tail of mice and blood glucose was observed before and after administration. Results: compared with the model control group, the serum lipids and hemorheology of the low and middle dose groups had the effect of reducing the blood glucose of diabetic foot mice (P0.01). There was a dose-dependent difference between the two groups (P0.05). The effect of high dose group was better than that of low dose group (P 0.05). Compared with the model control group, the high, medium and low dose groups had the effect of improving blood lipids and hemorheology (P0.05) after administration, and showed dose-dependent, the difference between the groups (P0.05). The effect of high dose group was better than that of low dose group in improving blood lipids and hemorheology. Conclusion: Corn whisker extract can effectively improve blood sugar, blood lipid and hemorheology of mice diabetic foot model. And the effect of improvement increased with the increase of dose concentration.
【作者单位】：黑龙江中医药大学附属第一医院;哈尔滨市第二医院;黑龙江中医药大学附属第二医院;
【基金】：黑龙江省教育厅科学技术研究项目(12541780)
【分类号】：R285.5;R-332
【正文快照】： 糖尿病足(Diabetic Foot,DF)是一种在糖尿病周围神经病变及外周血管病变基础上,由于足应力、外伤、感染等引起的严重下肢病变的综合症[1],是糖尿病较为多见的严重合并症之一,是一种慢性、进行性且累及大、中、小及微血管的病变,其最为常见的后果是慢性、反复性溃疡,最严重的结

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