流体剪切力对内皮细胞miR-21和miR-199a表达的影响

发布时间：2018-01-18 05:18

本文关键词：流体剪切力对内皮细胞miR-21和miR-199a表达的影响　出处：《生物信息学》2016年01期 　论文类型：期刊论文

更多相关文章： 剪切力 内皮细胞 microRNA 表达

【摘要】：为探讨流体剪切力对内皮细胞micorRNAs表达的影响。采用旋转锥形圆盘剪切力系统对内皮细胞分别加载低(4dyn/cm2)、中(10 dyn/cm2)和高(15 dyn/cm2)3种不同梯度的剪切力作用24h。对照组未加载剪切力。采用高通量筛选芯片检测microRNAs表达变化,qRT-PCR验证,并进行生物信息学分析。与对照组比较,低剪切力组表达差异的microRNAs有33个(FC1.5或0.5倍,P0.05),其中28个上调,5个下调;中剪切力组表达差异的microRNAs有8个(FC1.5或0.5倍,P0.05),其中6个上调,2个下调;高剪切力组表达差异的microRNAs有31个(FC1.5或0.5倍,P0.05),其中25个上调,6个下调。miR-21在高剪切力组中上调最显著(FC=0.026),在低剪切力组中显著下调(FC=3.531)。miR-199a在低剪切力组中上调最显著(FC=0.075),在高剪切力组中显著下调(FC=3.031)。表达差异的microRNA的靶基因主要与内皮细胞的力学信号转导、细胞跨膜迁移、钙离子信号通路、细胞内吞作用等相关。流体剪切力可诱导内皮细胞miR-21和miR-199a表达发生改变。
[Abstract]:In order to investigate the effect of fluid shear stress on the expression of micorRNAs in endothelial cells, a rotating conical disc shear stress system was used to load endothelial cells with 4 dyn / cm ~ (2), respectively. 10 dyn / cm ~ (2) and 15 dyn / cm ~ (2)). The shear stress of three different gradients was applied for 24 h, and the control group was not loaded with shear stress. High throughput screening microarray was used to detect the expression of microRNAs. Compared with the control group, 33 microRNAs with low shear stress were confirmed by qRT-PCR and analyzed by bioinformatics. 28 of them were up-regulated and 5 were downgraded; In the middle shear stress group, the expression of microRNAs was 1. 5 or 0. 5 times higher than that of the control group, among which 6 were up-regulated and 2 were down-regulated. In the high shear stress group, there were 31 microRNAs with FC1.5 or 0.5 times P0.05A, 25 of which were up-regulated. Six down-regulated .miR-21 were significantly up-regulated in high shear stress group (FC0.026). In the low shear stress group, the up-regulation of FCU 3.531 and miR-199a was significantly lower in the low shear stress group than that in the low shear stress group (0.075). In the high shear stress group, the target genes of the differentially expressed microRNA were significantly down-regulated. The target genes were mainly related to the mechanical signal transduction of endothelial cells, cell transmembrane migration and calcium signaling pathway. Fluid shear stress could induce changes of miR-21 and miR-199a expression in endothelial cells.
【作者单位】：山东省医学科学院基础医学研究所;济南大学山东省医学科学院医学与生命科学学院;山东省齐河县人民医院;
【基金】：国家自然科学基金项目(81470489) 山东省自然科学基金项目(ZR2014HM098) 山东省医药卫生科技发展计划项目(2014WS0312,2014WS0316)
【分类号】：R3416
【正文快照】： 血管内皮细胞(Endothelial cell,EC)是连接血流和血管壁的重要结构,是血流剪切力(Shearstress,SS)作用的靶细胞,其功能障碍是导致动脉粥样硬化形成的关键因素。研究表明[1-3],不同梯度的血流剪切力对内皮细胞的影响不同。microRNA是一类高度保守、内源性非蛋白编码小分子RNA,

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