人胚胎三叉神经节细胞形态发育研究

发布时间：2018-01-19 11:43

  本文关键词： 三叉神经节 人胚胎 节细胞 发育　出处：《新疆医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：观察人胚胎三叉神经节细胞形态结构及随胎龄增长变化发育规律。方法：取不同胎龄段人胚胎灌注固定后,外科显微镜下取双侧三叉神经节,常规HE染色,光镜观察；细胞计数；图像分析仪测量三叉神经节细胞直径、面积和周长；电镜下观察细胞内超微结构；比较不同胎龄时期细胞器变化情况；取1例标本在三叉神经根处植入DiI染色晶体,37℃恒温箱放置3个月,等待DiI染色晶体扩散,根据神经走向切片,荧光显微镜下观察神经元的形态;另取1例做冰冻切片神经元胞浆Nissl染色。结果：随着胎龄增长三叉神经节细胞数目无显著性变化；各胎龄组人胚胎三叉神经节神经细胞直径,19周至25周基本无变化,25周至32周发育过程中,平均直径增加最明显,面积和周长明显增大,与直径变化规律一致。电镜下,18-20周胎儿三叉神经节神经细胞排列紧密,胞质少,可见到数量不多的线粒体,且其内几乎未见嵴,其它细胞器较少。25周时,线粒体嵴变长,粗面内质网现雏形,有纵形小管出现;27周时可观察到成熟的高尔基复合体,32周时,线粒体、粗面内质网等细胞器结构发育趋于成熟；于33周电镜下可见溶酶体；36周时细胞内各种细胞器结构和功能基本完善；DiI追踪可清晰标记神经节细胞。结论：人胚胎三叉神经节细胞形态发育32周(8个月)时达成熟状态；三叉神经节细胞发育过程中随胎龄增加,其结构和功能逐步完善,32-36周(8-9月)是细胞的分化发育重要时期。
[Abstract]:Objective: to observe the morphological structure of trigeminal ganglion cells in human embryos and their developmental regularity with the increase of gestational age. Methods: the bilateral trigeminal ganglion was harvested under surgical microscope after different gestational age segments of human embryos were infused and fixed. Routine HE staining and light microscopic observation; Cell count; The diameter, area and perimeter of trigeminal ganglion cells were measured by image analyzer. The ultrastructure of the cells was observed under electron microscope. The changes of organelles at different gestational ages were compared. A specimen was implanted in a 37 鈩，

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