两种吸虫复染制片技术的探究

发布时间：2018-01-21 12:48

本文关键词： 华支睾吸虫布氏姜片吸虫单染法复染法　出处：《中国寄生虫学与寄生虫病杂志》2016年04期 　论文类型：期刊论文

【摘要】：采用醋酸卡红溶液和孔雀绿水溶液,对固定后的华支睾吸虫和布氏姜片虫标本进行复合染色。醋酸卡红对两种吸虫标本进行单染。复染法使得标本虫体呈三色,消化系统、排泄系统和周围肌肉组织均为浅红色,生殖器官中的子宫呈现鲜绿色,虫体两侧卵黄腺为黄褐色,虫体结构清晰、着色适度、立体感强。醋酸卡红染液单染对吸虫睾丸组织的显现力更强。在吸虫的教学和科研工作中,结合使用单染法和复染法,观察效果将会更好。
[Abstract]:The specimens of Clonorchis sinensis and turmeria brinsoni were stained with carmine acetate solution and malachite green solution. The specimens of two species of paragonimiasis were singly stained by carmine acetate, and the specimens were trichromatic by the method of restaining. The digestive system, excretory system and surrounding muscle tissue were all light red, the uterus in reproductive organs was bright green, the yolk glands on both sides of insect body were yellowish brown, the structure of insect body was clear and the staining was moderate. In teaching and scientific research of trematodes, the observation effect will be better if the method of single staining and double staining is combined with the method of single staining and double staining.
【作者单位】：皖南医学院医学影像与检验学院;皖南医学院医学寄生虫学教研室;
【基金】：皖南医学院大学生科研基金(No.WK2014S19)~~
【分类号】：R38
【正文快照】： 标本制作是寄生虫实验技术中基础而重要的工作。单染法常被应用于寄生虫学的教学和科研工作中[1-3],其制作技术已经比较成熟和普遍。复染法利用不同染料之间的性质差异,对不同组织的吸附性能不同,使得虫体各器官和组织之间产生色差,使虫体组织的立体感增强,便于观察。目前已有

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