新型序贯应激法致焦虑样胃高敏感性大鼠功能性消化不良模型的建立

发布时间：2018-01-24 02:29

本文关键词： 序贯应激法焦虑胃高敏感性功能性消化不良大鼠模型　出处：《中国实验动物学报》2017年05期 　论文类型：期刊论文

【摘要】：目的应用新型序贯应激法建立焦虑样胃高敏感性大鼠模型。方法将新生大鼠幼仔随机分为对照组与模型组,每组13只。出生后第2天起序贯采用胃刺激法、母婴分离法和束缚应激法制作焦虑样胃高敏感性模型。于第8周开始采用高架十字迷宫试验(EPM)、旷场试验(OF)、腹肌回缩试验(AWR)、肌电图试验(EMG)和胃排空试验(GET)等评价模型成功与否。结果 EPM和OF实验提示模型组大鼠较对照组表现出明显的焦虑样行为,AWR和EMG实验显示模型组大鼠对胃扩张刺激的敏感性亦显著升高(P0.05,P0.01),但两组在体重变化和胃黏膜炎症反应方面差异无显著性,符合胃敏感性增高为主功能性消化不良的临床特点。结论应用新型序贯应激法可成功建立焦虑样胃高敏感性大鼠模型。
[Abstract]:Objective to establish a high sensitivity rat model of anxiety stomach by a new sequential stress method. Methods newborn rats were randomly divided into control group and model group with 13 rats in each group. Gastric stimulation was used sequentially from the second day after birth. The high sensitivity model of anxiety stomach was made by mother and child separation method and restraint stress method. At the 8th week, the elevated cross labyrinth test was used to test EPMN, the open field test was OFN, and the abdominal muscle retraction test (AWR) was carried out. Electromyogram test (EMG) and gastric emptying test (Get) were used to evaluate the success of the model. Results EPM and of experiments showed that the model group showed more anxiety behavior than the control group. AWR and EMG experiments showed that the sensitivity of the model group to gastric expansion stimulation was also significantly increased, but there was no significant difference between the two groups in body weight change and gastric mucosal inflammation. Conclusion A new type of sequential stress method can be successfully used to establish an anxious gastric hypersensitive rat model.
【作者单位】：西安交通大学第二附属医院消化内科;延安大学附属宝鸡市人民医院消化内科;西安交通大学医学部药理学系;西安交通大学医学部基础医学实验教学中心;西安医学院第一附属医院病理科;
【分类号】：R-332;R57
【正文快照】： jun3z@163.com(1.Department of Gastroenterology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China;2.Department of Digestive Diseases,Baoji People’s Hospital Affiliated to Yan’an University,Baoji 721000;3.Department of Phar

【相似文献】