补气消癜汤对免疫性血小板减少症模型小鼠的白细胞介素系列因子及TNF-α的影响

发布时间：2018-01-25 02:33

本文关键词： 补气消癜汤免疫性血小板减少症白细胞介素肿瘤坏死因子-α　出处：《中华中医药学刊》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨补气消癜汤对免疫性血小板减少症模型(ITP)小鼠的白细胞介素系列因子及肿瘤坏死因子-α(TNF-α)的影响,旨在为临床治疗提供依据。方法:采用注射豚鼠抗小鼠血小板血清(GP-APS)方法建立ITP小鼠,随机将72只小鼠分为6组:空白组、模型组、强的松组、补气消癜汤组(低、中、高剂量),观察造模前、给药前、给药后小鼠外周血血小板计数(PLT);给药后,观察小鼠的脾脏系数、胸腺系数、骨髓巨核细胞数(MN)、脾脏MN,采用ELISA法测定小鼠血清白介素-2(IL-2)、IL-6、IL-10及肿瘤坏死因子-α(TNF-α)、血小板生成素(TPO)含量的变化。结果:1给药前,与空白组对比,模型组、强的松组、补气消癜汤各剂量组的PLT均出现明显下降(P0.01);给药后,各组小鼠的PLT较造模前减少,较给药前增多,差异显著(P0.01);与模型组对比,补气消癜汤各剂量组的PLT和TPO明显增多(P0.01)。2与空白组对比,模型组的脾脏系数、胸腺系数、脾脏MN、骨髓MN均明显增多(P0.01),与模型组对比,补气消癜汤各剂量组的脾脏系数、胸腺系数、脾脏MN、骨髓MN明显减少(P0.01或P0.05),与强的松组对比,补气消癜汤高剂量组的脾脏系数、胸腺系数、脾脏MN明显减少(P0.05)。3与空白组对比,模型组的IL-2、IL-6及TNF-α升高,IL-10降低,差异显著(P0.01);与模型组对比,强的松组、补气消癜汤各剂量组的IL-2、IL-6及TNF-α降低,IL-10升高,差异显著(P0.01);与强的松组对比,补气消癜汤高剂量组的IL-2及TNF-α降低,IL-10升高,差异显著(P0.01或P0.05)。结论:补气消癜汤可通过调节ITP小鼠体内IL-2、IL-6、IL-10及TNF-α分泌,促进脾脏和骨髓的巨核细胞分化,增加ITP模型小鼠外周血小板数。
[Abstract]:Objective: to investigate the effect of Buqi Xiao Schonlein decoction (BQXD) on interleukin series factor and tumor necrosis factor- 伪 (TNF- 伪) in mice with immune thrombocytopenia. Methods: ITP mice were established by injecting guinea-pig anti-platelet serum GP-APS. 72 mice were randomly divided into 6 groups: blank group and model group. Prednisone group, Buqi Xiao purpura decoction group (low, medium and high dose), observe the platelet count in peripheral blood of mice before, before and after administration; After administration, the spleen coefficient, thymus coefficient, bone marrow megakaryocyte number and spleen MNs were observed, and the serum interleukin-2 (IL-2) interleukin-6 (IL-6) was measured by ELISA method. The changes of IL-10 and TNF- 伪 TNF- 伪, thrombopoietin (TPO) contents were compared with those of blank group, model group and prednisone group. The PLT of each dose group of Buqi Xiaoxiao decoction decreased significantly (P 0.01). After administration, the PLT of mice in each group was lower than that before model making and increased compared with that before administration, and the difference was significant (P 0.01). Compared with the model group, the PLT and TPO of each dose group of Buqi Xiaoxiao decoction were significantly increased compared with the blank group, the spleen coefficient, thymus coefficient and spleen MN of the model group were significantly higher than those of the blank group. Compared with the model group, the spleen coefficient, thymus coefficient and spleen MN of each dose group of Buqi Xiao purpura decoction were significantly increased. Bone marrow MN significantly decreased P0.01 or P0.05, compared with prednisone group, the spleen coefficient and thymus coefficient of high dose group of Buqi Xiaoxiao decoction. Compared with the control group, the level of IL-2IL-6 and TNF- 伪 in the model group increased and IL-10 decreased significantly (P 0.01). Compared with the model group, the levels of IL-6 and TNF- 伪 in prednisone group and Buqi Xiao purpura decoction group decreased significantly (P 0.01). Compared with prednisone group, IL-2 and TNF- 伪 decreased the level of IL-10 in high dose group. Conclusion: Buqi Xiaopura decoction can regulate the secretion of IL-6 and IL-10 and TNF- 伪 in ITP mice. To promote the differentiation of megakaryocyte in spleen and bone marrow and to increase the number of peripheral platelets in ITP model mice.
【作者单位】：河南省中医院血液科;黄河科技学院医学院;
【基金】：河南省中医管理局重点专科(学科)学术带头人培养项目(121PCXTD510)
【分类号】：R285.5;R-332
【正文快照】： Influence of Interleukins Factors and TNF-αin Mice Model of ImmuneThrombocytopenia by Buqi Xiaodian DecoctionLIU Xianhui1,GUO Xiaona2免疫性血小板减少症(immune thrombocytopenia,ITP),又称为特发性血小板减少性紫癜,是临床所见血小板计数减少引起最常见出血性疾

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