IFN-γ抗鹦鹉热衣原体急性感染保护作用研究

发布时间：2018-01-25 04:39

本文关键词： 干扰素-γ 鹦鹉热衣原体急性感染免疫保护　出处：《中国人兽共患病学报》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨IFN-γ对鹦鹉热衣原体(Chlamydia psittaci,Cps)的抗感染作用,为进一步阐明机体抗衣原体免疫机制提供参考数据。方法不同浓度的重组人IFN-γ(5ng/mL、25ng/mL和50ng/mL)作用于感染Cps 6BC的HeLa细胞,48h后计数包涵体数量,并观察包涵体形态的改变。2×10~6 IFUs Cps 6BC滴鼻感染C57BL/6J小鼠,于感染前、后24h腹腔内注射10μg重组鼠IFN-γ,观察小鼠体重、活动状态、生存率等一般指标,分别于感染后5d和10d处死小鼠,取肝、肺组织进行HE染色检测其病理变化;并取感染后5d的肺组织,匀浆,计数其中Cps包涵体数量。结果感染48h后,Cps 6BC在5ng/mL、25ng/mL、50ng/mL重组人IFN-γ处理的HeLa细胞中包涵体数量低于对照组(包涵体数分别为(23.8±5.1)×10~6,(10±3.58)×10~6,(8.0±2.22)×10~6,(43.3±11.05)×10~6,衣原体包涵体形状不规则,体积变小。小鼠实验显示,腹腔注射IFN-γ可明显提高Cps 6BC感染小鼠生存率,并减轻急性临床表现及脏器病变。结论 IFN-γ可发挥早期抗Cps感染的保护作用。
[Abstract]:Objective to investigate the antiinfective effect of IFN- 纬 on Chlamydia psittacius (Cpss) of Chlamydia psittacius psittaci. Methods Recombinant human IFN- 纬 -5 ng / mL at different concentrations was used to further elucidate the mechanism of anti-chlamydia immunity. 25ng / mL and 50ng / mL were used to count the number of inclusion bodies in HeLa cells infected with Cps 6BC for 48 h. The morphological changes of inclusion bodies were observed. 2 脳 10 ~ (-6) IFUs Cps 6BC drops were used to infect C57BL / 6J mice before infection. After 24 hours, 10 渭 g recombinant IFN- 纬 was injected intraperitoneally to observe the weight, activity and survival rate of the mice. The mice were killed on the 5th and 10th day after infection, and the liver was taken out. The pathological changes of lung tissue were detected by HE staining. The number of Cps inclusion bodies was counted in lung homogenate 5 days after infection. Results after 48 hours of infection, the number of CCS 6BC was 25 ng / mL at 5 ng / m L ~ (-1) ~ (-1) ~ (-1) 路min ~ (-1) 路L ~ (-1) 路L ~ (-1). The number of inclusion bodies in HeLa cells treated with 50 ng / mL recombinant human IFN- 纬 was lower than that in control group (23.8 卤5.1) 脳 10 ~ (-6). The shape of chlamydia inclusion body is irregular, 10 卤3.58 脳 10 ~ (6) + 8. 0 卤2.22) 脳 10 ~ (6) ~ (6) 脳 10 ~ (3) 卤11.05) 脳 10 ~ (6). The shape of inclusion body of Chlamydia is irregular. Mice experiment showed that intraperitoneal injection of IFN- 纬 could significantly improve the survival rate of mice infected with Cps 6BC. Conclusion IFN- 纬 may play an early protective role against Cps infection.
【作者单位】：南华大学医学院病原生物学研究所;广州市第八人民医院传染病研究所;中山市人民医院检验医学中心;
【基金】：国家自然科学基金(No.31270218) 中山市卫生局科研基金(No.J2012002) 中山市科技计划项目(No.2015B1025)联合资助~~
【分类号】：R374.2
【正文快照】： 鹦鹉热衣原体(Chlamydia psittaci,C.psitta-ci,Cps)为革兰阴性胞内寄生菌,主要感染家禽和宠物鸟,可经气溶胶吸入或密切接触传染给人[1-2]。依据Cps菌株和宿主类型的不同,其临床疾病表现多种多样。禽类和牲畜类主要表现为肺炎和慢性肠炎表现,人类主要表现为呼吸系统感染,也可

【相似文献】