金丝桃苷对D-半乳糖衰老模型小鼠的抗衰老作用

发布时间：2018-01-25 06:44

本文关键词： 金丝桃苷 D-半乳糖衰老模型抗衰老　出处：《中药药理与临床》2017年01期 　论文类型：期刊论文

【摘要】：目的:研究金丝桃苷对D-半乳糖所致衰老模型小鼠的抗衰老作用。方法:采用D-半乳糖建立小鼠衰老模型,同时实验组分别灌胃不同剂量(25、50、100 mg/kg)的金丝桃苷,通过八臂迷宫试验和游泳耐力测验检测衰老模型小鼠短期学习记忆能力和抗疲劳能力。随后处死小鼠,采用ELISA法检测小鼠脑总抗氧化能力(T-AOC),超氧化物岐化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)、活性氧(ROS)和丙二醛(MDA)含量,通过Western blot测定沉默信息调节因子1(Sirt1)、核因子-E2-相关因子(Nrf2)和醌氧化还原酶(NQO1)蛋白表达。结果:与模型组比较,金丝桃苷(25~100 mg/kg)小鼠游泳耐力更好,迷宫通过时间缩短、错误次数减少,脑组织T-AOC、SOD、GSH-Px活性明显升高,而脑组织ROS和MDA含量显著降低,并上调Sirt1、Nrf2和NQO1蛋白表达。结论:金丝桃苷对D-半乳糖致衰老小鼠具有抗氧化作用,其作用可能通过调控Sirt1/Nrf2信号通路相关。
[Abstract]:Objective: to study the anti-aging effect of hyperin on aging model mice induced by D-galactose. The short-term learning and memory ability and anti-fatigue ability of aging model mice were tested by eight-arm maze test and swimming endurance test with 100 mg / kg hyperin. Then the mice were killed. The activity of T-AOCX, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured by ELISA method. The contents of Ros) and malondialdehyde (MDA) were determined by Western blot. Results: compared with the model group, the expression of nuclear factor-E2-related factor nrf2) and quinone redox enzyme NQO1) protein. Hyperoside 25 mg / kg mice had better swimming endurance, shorter maze passing time, fewer errors, and higher GSH-Px activity in brain tissue. However, the contents of ROS and MDA in brain tissue were significantly decreased, and the expression of rf2 and NQO1 protein of Sirt1nrf2 were up-regulated. Conclusion: hyperoside has antioxidation effect on aging mice induced by D-galactose. Its role may be related to the regulation of Sirt1/Nrf2 signaling pathway.
【作者单位】：遵义医学院药学院;辽宁中医药大学基础医学院;
【基金】：贵州省科学技术基金联合基金项目(遵义医学院2015年度三方合作协议项目)黔科合LH字[2015]7540号
【分类号】：R285.5;R-332
【正文快照】： 衰老是指人体细胞及组织在结构和机能上所出现的退行性变化现象,是生物生命过程中的必然规律。主要表现为器官重量减轻,细胞萎缩,胞质色素沉着,功能代谢降低,适应能力减弱,抗病能力低下等[1]。衰老的研究成为当今的一个研究热点和难点问题[2]。中药基于作用的多靶点,在衰老的

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