鱼蛋白胨的制备与质量分析

发布时间：2018-01-25 11:36

  本文关键词： 鱼蛋白胨 质量分析　出处：《中国生物制品学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的制备鱼蛋白胨,并进行质量分析。方法将鱼糜加工下脚料原料经绞碎、石油醚脱脂,得到鱼糜浆,用0.1%木瓜蛋白酶酶解,离心收集上清(即鱼水解蛋白胨溶液),经300 Da纳滤膜脱盐和浓缩,喷雾干燥后,获得淡黄色粉末,即鱼蛋白胨样品FZU01。对鱼蛋白胨FZU01和鱼粉蛋白的主要理化指标(澄明度、亚硝酸盐和碱性沉淀、酸碱度、含氮量、氨基氮、干燥失重、炽灼残渣)进行检测;在固定酵母提取物、Na Cl浓度、初始p H和接种量的基础上,分别用两种蛋白胨配制LB培养基,接种大肠埃希菌,检测A600值;采用分子筛高效液相色谱法(size-exclusion highperformance liquid chromatography,SEC-HPLC)检测两种蛋白胨的相对分子质量。分别将50、150、300、600、900、1 200和1 500 mg/L组胺以及0.1、0.5、1.0、5.0、10.0、20.0、30.0、40.0和50.0 mg/L重金属离子(Hg2+、Cd2+、As3+、Cr6+和Pb2+)加至大肠埃希菌中,37℃培养12 h,检测A600值。结果两种蛋白胨理化指标检测结果均符合《中国生物制品主要原辅材料质控标准》规定,酸碱度、含氮量、氨基氮、干燥失重和炽灼残渣之间差异无统计学意义(P0.05),鱼蛋白胨FZU01相对分子质量主要分布在1 000以内,占92.5%,鱼粉蛋白胨相对分子质量主要分布在10 000,小于1 000占56.1%;与鱼粉蛋白胨组相比,鱼蛋白胨FZU01组生长速率明显增加(P0.05)。随着组胺浓度的增加,大肠埃希菌的生长抑制率逐渐增加,与组胺浓度呈正相关,IC50为257.3 mg/L,当组胺浓度大于600 mg/L时,完全抑制大肠埃希菌的生长;大肠埃希菌的生长抑制率与重金属离子浓度呈正相关,Hg~(2+)、Cd~(2+)、As~(3+)、Cr~(6+)和Pb~(2+)对大肠埃希菌的IC50分别为1.35、4.67、7.37、11.04和20.19 mg/L。结论应增加蛋白质相对分子质量、组胺和重金属作为蛋白胨产品重要质量参数,控制一定的浓度限量范围,才能保证蛋白胨产品的稳定性和安全性。
[Abstract]:Objective to prepare fish peptone and analyze its quality. Methods the raw material of fish surimi was chopped and degreased with petroleum ether, and the surimi was hydrolyzed with 0.1% papain. The supernatant (i.e. fish hydrolyzed peptone solution) collected by centrifugation was desalinated and concentrated by 300Da nanofiltration membrane and then spray-dried to obtain yellowish powder. Fish peptone sample FZU01. The main physicochemical indexes of fish peptone FZU01 and fish meal protein (clarity, nitrite and alkaline precipitation, pH, nitrogen content, amino nitrogen, dry weight loss). Incandescent residue); On the basis of fixed yeast extract NaCl concentration, initial pH and inoculation amount, LB medium was prepared with two kinds of peptone, inoculated with Escherichia coli, and A600 value was detected. Molecular sieve high performance liquid chromatography (HPLC) was used for size-exclusion highperformance liquid chromatography. The relative molecular weight of two peptone was determined by SEC-HPLC. The histamine and histamine of 500 mg/L and 1 500 mg/L histamine were determined, respectively. The heavy metal ions Hg2 and CD2 as _ 3 of 0.50 ~ 1.0 ~ 10.0 ~ (10.0) ~ 20.0 ~ (30) ~ (30) and 50 ~ (0) mg/L heavy metal ions Hg2 ~ (2 +) ~ (2 +). Cr6 and Pb2 were added to Escherichia coli at 37 鈩，

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