香鳞毛蕨有效部位乳膏对糠秕马拉色菌所致豚鼠感染模型的影响

发布时间：2018-01-25 15:25

本文关键词： 香鳞毛蕨有效部位乳膏体内抗真菌活性糠秕马拉色菌豚鼠模型　出处：《中国实验方剂学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:评价香鳞毛蕨有效部位乳膏(DF乳膏)对糠秕马拉色菌所致豚鼠感染模型的治疗作用。方法:构建糠秕马拉色菌豚鼠皮肤感染模型,按皮损评分值随机分成模型组,酮康唑组(1.33 mg·g-1),DF乳膏高、中、低剂量组(含生药2,1,0.5 g·g-1),通过测定肉眼评分、菌落计数(CFU),真菌学转阴率及组织病理检查4项指标,评价DF乳膏对糠秕马拉色菌所致豚鼠感染模型的治疗作用。结果:与模型组比较,DF乳膏高、中、低剂量组均可显著降低糠秕马拉色菌所致感染模型豚鼠皮损评分(P0.05),明显减少病变皮肤菌落形成数(P0.05),提高真菌培养的转阴率(P0.05,P0.01);皮肤病理切片结果表明DF乳膏高、中剂量可明显减少感染组织皮肤角质层孢子数。结论:DF乳膏对糠秕马拉色菌所致的豚鼠感染有较好的治疗作用。
[Abstract]:Objective: to evaluate the therapeutic effect of extract DF cream of Dryopteris furfurum on guinea pig infected with Malassezia furfurum. Methods: the skin infection model of Malassezia furfurum in guinea pigs was established. According to the score of skin lesion, ketoconazole group was randomly divided into three groups: the ketoconazole group was 1.33 mg 路g-1D ointment with high, middle and low doses (containing 21g 路g ~ (-1)) crude drug. Four indexes including gross score, colony count, mycological negative rate and histopathological examination were measured. To evaluate the therapeutic effect of DF cream on guinea pig infection induced by Malassezia furfurum. Results: compared with the model group, DF cream was higher and higher than that of the model group. The low dose group could significantly reduce the skin lesion score of guinea pigs induced by Malassezia furfurum (P0.05), and significantly reduce the number of diseased skin colony formation (P0.05). To increase the negative rate of fungi culture. The results of pathological section of skin showed that DF cream was high and medium dose could obviously reduce the number of spores in the cuticle of infected tissues. Conclusion the solution of 10% DF cream has a better therapeutic effect on the infection caused by Malassezia furfurum in guinea pigs.
【作者单位】：广东药科大学中药学院实验动物中心;
【基金】：国家中医药管理局公益性行业科研专项(201507004) 国家“重大新药创制”科技重大专项(2011ZX09102-007-03) 广东省科技计划项目(2015B020234009) 广东省公益研究与能力建设专项资金项目(2015A030302088)
【分类号】：R275;R-332
【正文快照】： [网络出版地址]http://www.cnki.net/kcms/detail/11.3495.R.20161129.1058.018.html[网络出版时间]2016-11-29 10:58糠秕马拉色菌Malassezia furfur,属于马拉色菌属中常见的皮肤寄生菌致病菌。从马拉色菌属所致的各类型疾病中均能分离出M.furfur[1],该菌主要分布于人的头皮、

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