电针对心肌缺血模型大鼠氧化应激与缝隙连接蛋白43的影响

发布时间：2018-01-25 23:46

本文关键词： 心肌缺血针刺疗法标本配穴缝隙连接蛋白　出处：《中华中医药杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:观察"标本配穴"电针对慢性心肌缺血大鼠缺血心肌缝隙连接蛋白43(Cx43)表达的影响,探讨"标本配穴"电针防治心肌缺血的作用机制。方法:采用SPF级雄性Wistar大鼠60只,随机分为4组:正常组、模型组、内关治疗组、标本配穴治疗组,每组15只。除正常组连续14d皮下注射0.9%氯化钠溶液外(2mg·kg~(-1)·d~(-1)),其他3组均每日给予盐酸异丙肾上腺素(2mg·kg~(-1)·d~(-1))皮下注射,每日1次,连续14d。两个治疗组在造模开始当天即给予电针针刺治疗,正常组和模型组相同时间抓取固定,每日1次,共21d。采用ELISA检测各组大鼠MDA、SOD、VCAM-1,Western blot及免疫组化法检测Cx43表达及分布。结果:与正常组比较,模型组MDA、VCAM-1含量升高,总SOD活力、心肌Cx43蛋白表达降低(P0.05);与模型组比较,内关治疗组、标本配穴组MDA、VCAM-1含量降低,总SOD活力、心肌Cx43蛋白表达升高(P0.05);标本配穴组总SOD活力、VCAM-1含量均低于内关治疗组,Cx43蛋白表达高于内关治疗组(P0.05)。结论:电针可通过促进Cx43的表达,改善其分布,保护或者修复缝隙连接使其发挥保护心肌的作用;"标本配穴"电针可更好的通过促进Cx43的表达和改善心肌Cx43的分布,起到保护慢性心肌缺血模型大鼠心肌的作用。
[Abstract]:Objective: to observe the effect of electroacupuncture on the expression of gap junction protein 43 (Cx43) in ischemic myocardium of rats with chronic myocardial ischemia. Methods: 60 SPF grade male Wistar rats were randomly divided into 4 groups: normal group, model group and Neiguan treatment group. The specimens were treated with acupoints, 15 rats in each group. 0.9% sodium chloride solution was subcutaneously injected subcutaneously for 14 days in the normal group (2 mg 路kg ~ (-1)) 路d ~ (-1)). The other three groups were given isoproterenol (2mg 路kg ~ (-1)) 路dang ~ (-1) subcutaneously daily, once a day. For 14 days, the two groups were treated with electroacupuncture on the day when the model was made, and the normal group and the model group were fixed at the same time, once a day. For 21 days, ELISA was used to detect VCAM-1 in rats. Western blot and immunohistochemical method were used to detect the expression and distribution of Cx43. Results: compared with the normal group, the MDA-VCAM-1 content in the model group increased and the total SOD activity increased. The expression of Cx43 protein in myocardium decreased P0.05A; Compared with the model group, the content of MDA-VCAM-1, the activity of total SOD and the expression of Cx43 protein in myocardium increased in Neiguan treatment group. The expression of total SOD activity and VCAM-1 in the sample matching group was lower than that in the Neiguan treatment group, and the expression of Cx43 protein was higher than that in the Neiguan treatment group (P 0.05). Conclusion: electroacupuncture can promote the expression of Cx43. Improve its distribution, protect or repair gap junction to play a role in myocardial protection; The electroacupuncture can improve the expression of Cx43 and improve the distribution of myocardial Cx43, which can protect the myocardium of chronic myocardial ischemia rats.
【作者单位】：湖北中医药大学/针灸治未病湖北省协同创新中心;
【基金】：国家自然科学基金项目(No.81473789,No.81273865,No.81403460)~~
【分类号】：R245;R-332
【正文快照】： 目前研究发现闰盘中的缝隙连接(gap junction,GJ)与心脏的正常传导和引起心律失常的异常传导相关[1],缝隙连接蛋白43(connexion 43,Cx43)作为缝隙连接中最主要的蛋白,密切参与了心肌缺血、心肌梗死甚至于心力衰竭的病理生理过程,也就是说Cx43的重构与心肌缺血的发病机制直接有

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