卵白蛋白介导白毛黑眼兔变应性鼻炎模型树突状细胞功能的改变

发布时间：2018-01-28 01:02

本文关键词： 卵白蛋白变应性鼻炎 WHBE兔树突状细胞　出处：《中国实验动物学报》2017年03期 　论文类型：期刊论文

【摘要】：目的比较观察卵白蛋白(OVA)诱导的白毛黑眼兔(WHBE兔)和日本大耳白兔(JW兔)变应性鼻炎(allergic rhinitis,AR)模型外周血来源树突状细胞(dendritic cells,DC)的功能,揭示WHBE兔对AR敏感的可能机制。方法以OVA诱导建立WHBE兔和JW兔AR模型,每次激发后观察动物一般体征,进行鼻黏膜HE染色,观察组织病理学改变。分离外周血DC,流式细胞仪检测外周血DC表面分子CD86的表达以及DC摄取抗原的能力,同时,采用荧光定量PCR检测外周血DC甘露糖受体(mannose receptor,MR)的mRNA表达水平。进一步以CFSE标记T细胞,流式细胞仪检测外周血DC诱导T细胞增殖能力。结果一般体征和HE染色观察显示,WHBE兔和JW兔模型组动物均表现为典型的变应性鼻炎症状和组织病理学改变,提示模型构建成功。WHBE兔AR模型组外周血DC CD86的表达不仅极显著高于正常对照组,亦极显著高于JW兔AR模型组(P0.01,P0.01)。正常稳态下,WHBE兔外周血DC MR的mRNA相对表达量极显著高于JW兔(P0.01),经OVA诱发建立AR模型,WHBE兔外周血DC MR的mRNA表达水平显著升高(P0.05),且显著高于JW兔模型组(P0.05)。并且,WHBE兔模型组外周血DC摄取OVA647的能力极显著高于正常对照组(P0.01),亦极显著高于JW兔模型组(P0.01)。结论 WHBE兔DC对变应原更敏感,可能与其高表达抗原识别受体MR,具备更强的分化成熟、摄取抗原能力有关。
[Abstract]:Objective to observe the allergic rhinitis induced by ovalbumin (OVA) in white haired black eyed rabbits (WHBE) and Japanese large ear white rabbits (JW). The function of dendritic cells derived from peripheral blood derived from dendritic cells (DC). Methods the AR models of WHBE rabbits and JW rabbits were induced by OVA. The general signs of the animals were observed and nasal mucosa HE staining was performed after each stimulation. The changes of histopathology were observed. The expression of CD86 on the surface of peripheral blood DC and the ability of DC to absorb antigen were detected by flow cytometry. The expression of mRNA in peripheral blood DC mannose receptor MRs was detected by fluorescence quantitative PCR, and T cells were further labeled with CFSE. The proliferation ability of T cells induced by peripheral blood DC was detected by flow cytometry. Results the general signs and HE staining were observed. WHBE rabbits and JW rabbits showed typical allergic rhinitis symptoms and histopathological changes. The results suggested that the expression of DC CD86 in the model group was significantly higher than that in the normal control group and JW rabbit AR model group (P0.01). The relative expression of mRNA in peripheral blood DC Mr was significantly higher in white rabbits than that in JW rabbits under normal steady-state condition. The AR model was induced by OVA. The mRNA expression of DC Mr in peripheral blood of WHBE rabbits was significantly higher than that of JW rabbit model group. The ability of DC uptake of OVA647 in WHBE rabbit model group was significantly higher than that in normal control group (P0.01). Conclusion WHBE rabbit DC is more sensitive to allergen than JW rabbit model group, and may have stronger differentiation and maturity with high expression of antigen recognition receptor MRR. The ability to ingest antigen is related.
【作者单位】：浙江中医药大学动物实验研究中心/比较医学研究所;浙江中医药大学基础医学院;
【基金】：浙江省科技计划项目(批准号:2014C37007) 浙江中医药大学比较医学创新团队(XTD201301)
【分类号】：R-332;R765.21
【正文快照】： ges.Expressions of CD86 on the cell surface of dendritic cells in WHBE rabbits with AR were significantly upregulated notonly compared with the normal control(NC)rabbits,but also with the JW rabbits with AR(P0.01).The result of real-time PCR assay showe

【相似文献】