不同海拔和不同持续时间大鼠血浆3种白介素水平的变化

发布时间：2018-01-28 17:56

本文关键词： 大鼠不同海拔不同持续时间白介素　出处：《动物医学进展》2017年05期 　论文类型：期刊论文

【摘要】：测定不同海拔和不同持续时间大鼠血浆IL-6、IL-8和IL-10水平,探讨海拔和时间对大鼠血浆3种白介素含量变化的影响。选用88只清洁雄性Wistar大鼠随机分为对照组、中海拔组和高海拔组。对照组立即抽取下腔静脉血3mL分离血浆保存,中、高海拔组饲养1、3、7、15、30d后抽取下腔静脉血3mL分离血浆保存,ELISA测定血浆白介素水平。结果表明,中海拔1、3、7天组IL-6、IL-8水平均高于对照组(P0.05);高海拔组中不同时间组IL-6、IL-8水平均高于对照组(P0.05),第7天上升至最高后呈下降,但仍高于对照组;中海拔组IL-10水平上升后呈下降趋势,不同时间组仍均高于对照组(P0.05);IL-10水平在高海拔组中不同时间组均低于对照组(P0.05),并随时间下降;IL-10水平在高海拔组同一时间组中均低于中海组同一时间组(P0.05)。上述结果提示,中海拔可导致机体内出现炎症反应,7d后逐渐下降至初始水平;高海拔环境中机体炎性反应增强,7d后逐渐减轻,但持续存在炎症及免疫抑制反应。
[Abstract]:The levels of IL-8 and IL-10 in plasma of rats with different altitude and duration were measured. To investigate the effect of altitude and time on plasma levels of three kinds of interleukin in rats 88 clean male Wistar rats were randomly divided into control group. In the middle altitude group and the high altitude group, 3 mL of inferior vena cava blood was taken from the control group immediately, and 3 mL of the inferior vena cava blood was collected from the high altitude group for 30 days. The results showed that the levels of IL-6 and IL-8 in the middle altitude group were higher than those in the control group (P 0.05). The level of IL-6 IL-8 in the high altitude group was higher than that in the control group at different time points (P 0.05). The level of IL-8 increased to its highest level on the 7th day, but it was still higher than that of the control group. The level of IL-10 in middle altitude group was decreased after rising, and it was still higher in different time groups than that in control group (P 0.05). The level of IL-10 in the high altitude group was lower than that in the control group at different time and decreased with time. The level of IL-10 in the same time group of high altitude group was lower than that in the same time group of Zhonghai group. After 7 days, it gradually decreased to the initial level. The inflammatory response in high altitude environment decreased gradually after 7 days, but there were persistent inflammatory and immunosuppressive reactions.
【作者单位】：青海省人民医院;青海大学;
【基金】：青海省科技厅应用基础研究项目(2013-Z-741) 青海省重点学科项目(呼吸科)(青财社字[2013]2578号)
【分类号】：R363
【正文快照】： 目前,国内外关于低氧对机体的损伤的报道很多,而多数为低氧诱导因子等氧化/抗氧化通路相关研究及进展。近来报道指出,高氧暴露或低氧均具有致炎作用,能够激活炎性细胞、促进炎性因子释放、急性反应蛋白水平上升等[1-2]。在炎症反应中,白介素作为重要的炎性介质,成为炎症因子的

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