IL-37对人原代髓样树突状细胞炎症相关因子表达的影响

发布时间：2018-01-30 05:29

本文关键词： 白细胞介素髓样树突状细胞流式细胞术肿瘤坏死因子α 白细胞介素白细胞介素　出处：《山东医药》2017年42期 　论文类型：期刊论文

【摘要】：目的观察白细胞介素37(IL-37)对人原代髓样树突状细胞(m DC)表达炎症相关因子TNF-α、IL-6、IL-12的影响。方法使用淋巴细胞分离液将全血细胞分离后提取单个核细胞,流式细胞分选方法分离纯化CD11c+CD123lowHLA-DR+的m DC。将分选后的m DC分为3组:未处理组加入完全培养基;对照组加入完全培养基培养后,再加入100 ng/mL LPS;IL-37预处理组分别加入0.125、0.25、0.5、1μg/mL IL-37后,再加入100 ng/mL LPS。采用实时荧光定量PCR方法检测m DC中TNF-α、IL-6、IL-12 mRNA表达,ELISA法检测细胞上清液中TNF-α、IL-6、IL-12水平。结果与未处理组比较,对照组TNF-α、IL-6、IL-12 mRNA表达均升高(P均0.01)。与对照组相比,IL-37预处理组加入0.25、0.5、1μg/mL IL-37预处理后,TNF-α、IL-6 mRNA表达降低;加入0.5、1μg/mL IL-37预处理后,IL-12 mRNA表达降低(P均0.01)。与未处理组比较,对照组细胞上清液中TNF-α、IL-6、IL-12水平升高。各浓度IL-37预处理组与对照组相比,TNF-α水平均降低(P均0.05);加入0.25μg/mL IL-37预处理后,细胞上清液中IL-6、IL-12水平降低(P均0.05)。结论 IL-37能够降低人原代m DC表达和分泌炎症相关因子。
[Abstract]:Objective to observe the effect of interleukin-37 (IL-37) on the expression of TNF- 伪 IL-6 in human primary myeloid dendritic cells (MDC). Effect of IL-12. Methods Mononuclear cells were isolated from whole blood cells with lymphocytes. CD11c CD123lowHLA-DR was purified by flow cytometry. The mDC was divided into three groups: the untreated group was added to the complete culture medium; In the control group, 100 ng/mL LPS was added to the culture medium. In IL-37 preconditioning group, 0.125 渭 g / mL IL-37 was added to 0.125 渭 g / mL 0.25 渭 g / mL IL-37. The expression of IL-12 mRNA in mDC was detected by real-time fluorescence quantitative PCR. The level of IL-12 in the supernatant of TNF- 伪 was detected by ELISA. Results compared with the untreated group, TNF- 伪 IL-6 was detected in the control group. The expression of IL-12 mRNA was increased (P < 0.01). Compared with the control group, the IL-37 preconditioning group was pretreated with 0.25 渭 g / mL IL-37. The expression of IL-6 mRNA in TNF- 伪 was decreased. After pretreatment with 0.5 渭 g / mL IL-37, the expression of IL-12 mRNA decreased (P < 0.01). Compared with the untreated group, TNF- 伪 in the supernatant of the control group. The levels of IL-6 and IL-12 increased, and the levels of TNF- 伪 decreased in each IL-37 pretreatment group compared with the control group (P < 0.05). After pretreatment with 0.25 渭 g / mL IL-37, IL-6 in the supernatant was obtained. Conclusion IL-37 can decrease the expression and secretion of inflammatory cytokines in primary human DC.
【作者单位】：天津市东丽区东丽医院;天津市河西区陈塘庄街社区卫生服务中心;
【基金】：天津市东丽区科技发展计划(2015DL10002)
【分类号】：R392
【正文快照】： 陈塘庄街社区卫生服务中心)髓样树突状细胞(m DC)是一群专职的抗原提呈细胞,它能够捕获、加工并通过其表面受体提呈抗原给T细胞。m DC是适应性免疫反应和固有免疫反应的桥梁[1]。白细胞介素37(IL-37)在人类体内大多数类型的细胞中均有表达,包括单核细胞、树突状细胞、内皮细胞

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