黎族人肠道微生物群落结构特征及其与饮食关联性

发布时间：2018-01-30 17:53

本文关键词： 黎族肠道菌群饮食高通量测序　出处：《微生物学通报》2017年11期 　论文类型：期刊论文

【摘要】：【目的】对采集自海南省白沙地区的黎族健康志愿者肠道菌群进行研究,旨在揭示黎族人肠道微生物群落结构特征及其与饮食的相关性。【方法】以海南省白沙黎族自治县征集的22名志愿者晨便为研究对象,应用基于16S r RNA基因V3 V4可变区的高通量测序技术测定其肠道菌群组成,并与其他民族肠道菌群进行比较分析,详细记录黎族22名志愿者的营养物质摄入情况,探索其肠道微生物群落结构特征及其与饮食的相关性。【结果】在门水平上,拟杆菌门(Bacteroidetes,58.96%)和硬壁菌门(Firmicutes,37.77%)在黎族志愿者肠道内含量最高;在属的水平上,普氏菌属(Prevotella,49.38%)在黎族健康志愿者肠道内含量最高。基于微生物群落α和β多样性的分析结果表明,黎族人肠道菌群与中国其他民族人群肠道菌群呈现出显著差异且α多样性显著低于其他民族,特征性差异菌属为:链型杆菌属(Catenibacterium)、普氏菌属(Prevotella)、巨型球菌属(Megasphaera)、巨单胞菌属(Megamonas)、考拉杆菌属(Phascolarctobacterium)和布劳特氏菌属(Blautia)。基于肠道核心微生物与营养物质相关性的研究显示,普氏粪杆菌(Faecalibacterium prausnitzii)与膳食纤维、Cu、Mg和Mn的摄入量呈现显著正相关,与脂肪和VB2的摄入量呈现显著负相关,而罗氏乳杆菌(Lactobacillus rogosae)与膳食纤维、Zn和Fe的摄入量呈显著正相关,与烟酸摄入量呈显著负相关。【结论】揭示了肠道微生物在不同地域和民族之间的差异,研究结果提供了一种通过膳食来优化菌群结构、调控宿主肠道微生态平衡的新思路。
[Abstract]:[objective] to study the intestinal flora of Li nationality healthy volunteers collected from Baisha area, Hainan Province. The aim of this study was to reveal the characteristics of intestinal microbial community and its correlation with diet. [methods] 22 volunteers collected from Baisha Li Autonomous County of Hainan Province were selected as the study objects. The composition of intestinal flora was determined by high-throughput sequencing based on the variable region of V3 / V4 of 16s r RNA gene, and compared with that of other nationalities. The nutrient intake of 22 volunteers of Li nationality was recorded in detail to explore the characteristics of intestinal microbial community structure and its correlation with diet. [results] at gate level. Bacteroidetes58.96) and Firmicutes37.77) were the highest in the intestinal tract of Li nationality volunteers. At the genus level, Prevotellaus 49.38) was the highest in the intestinal tract of healthy volunteers of Li nationality. The analysis results based on the diversity of microbe community 伪 and 尾 showed that. The intestinal flora of Li nationality was significantly different from that of other ethnic groups in China, and the 伪 -diversity was significantly lower than that of other nationalities. The characteristic differential genera are: Streptococcus, Catenibacterium, Prevotellaa, Megasphaera. Megacomonas (Megamonas). Koalas (Phascolarctobacter) and Blauterium (Blauterium). Based on the study of the relationship between intestinal core microbes and nutrients. Faecalibacterium prausnitzii was positively correlated with the intake of Cu, mg and mn in dietary fiber. There was a significant negative correlation with the intake of fat and VB2, but there was a significant positive correlation between Lactobacillus rogosaeae and dietary fiber intake of Zn and Fe. There was a significant negative correlation with the intake of nicotinic acid. [conclusion] the differences of intestinal microbes in different regions and nationalities were revealed, and the results provided a way to optimize the flora structure through diet. A new idea for regulating the microecological balance of the host intestine.
【作者单位】：海南大学食品学院;
【基金】：海南省自然科学基金面上项目(No.20163042) 海南大学科研启动基金(No.KYQD1548)~~
【分类号】：R37
【正文快照】： 人体肠道中栖息着约30个属500多种动态细菌,这些细菌的动态变化在维持人体健康方面起着不可替代的作用,它们与人体形成了密不可分的互惠共生关系[1]。研究发现,肠道微生物在不同结构和组成上会影响宿主的营养物质加工、能量代谢平衡[2]、免疫发育和成熟[3]及其它多种重要的生

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