控制挫伤深度建立大鼠颈脊髓半侧挫伤模型

发布时间：2018-01-31 21:13

本文关键词： 颈脊髓损伤半侧挫伤前肢运动功能大鼠组织学　出处：《中国脊柱脊髓杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的 :建立大鼠颈脊髓半侧挫伤模型,评价不同程度脊髓损伤大鼠的行为学和组织学改变。方法 :选取32只成年雄性Sprague-Dawley大鼠,体重为280~320g。按挫伤深度分为1.2mm组(n=12)和2mm组(n=12),并设立假手术组(n=8)对照。麻醉状态下后路手术暴露大鼠C5左侧脊髓,特制椎夹固定颈椎并连于大鼠立体定位架后置于脊髓打击器平台上,然后应用直径为1.5mm的圆柱形打击头在500mm/s的速度下分别以1.2mm和2mm打击深度背侧垂直挫伤大鼠C5脊髓左半侧,监测最大打击力和挫伤能量。每只大鼠损伤后进行前肢运动功能评分,圆柱攀爬试验和梳理试验。伤后8周取材,在脊髓横断面组织学切片上测量损伤中心处脊髓损伤面积,以及灰、白质残存面积。结果:中度损伤组最大打击力和挫伤能量分别为0.99±0.14N和(1.08±0.01)×10-5J,重度损伤组为1.53±0.22N和(8.56±0.02)×10-5J,两组间打击力和损伤能量具有统计学差异(P0.05)。术后对照组大鼠均未见明显神经功能障碍,前肢运动功能评分为16.29±0.49。损伤组大鼠均出现单侧神经功能障碍,损伤同侧前肢运动功能评分中度损伤组为1.40±0.52,重度损伤组为0.83±0.25,两组无统计学差异(P0.05)。伤后8周前肢运动功能评分中度损伤组为12.40±1.15,重度损伤组为8.41±0.83,对照组为17.00±0.00,梳理试验评分中度损伤组为3.80±0.60,重度损伤组为2.51±0.54,对照组为5.00±0.00,损伤同侧前肢使用比例中度损伤组为(32.31±5.70)%,重度损伤组为(4.92±1.21)%,对照组为(49.42±1.20)%,各组间均有统计学差异(P0.05)。镜下观察损伤组大鼠脊髓组织都出现了明显的半侧空洞和结构破坏,而对侧脊髓结构基本完整。在损伤中心处,中度损伤组脊髓背侧束、背外侧束和脊髓后角有破坏,重度损伤组脊髓仅残留部分的腹外侧束,对照组脊髓组织结构完整。灰质和白质残存面积在中度损伤组分别为1.45±0.11mm~2和4.13±0.29mm~2,重度损伤组分别为1.10±0.05mm~2和3.84±0.19mm~2,组间均存在统计学差异(P0.05)。结论:本研究成功建立了不同程度的大鼠颈脊髓半侧挫伤模型,不同挫伤深度表现出不同程度的单侧前肢运动功能障碍和脊髓组织破坏,为颈脊髓半侧损伤模型提供研究基础。
[Abstract]:Objective: to establish a rat model of cervical spinal cord contusion. Methods: 32 adult male Sprague-Dawley rats were selected. The body weight was 280 ~ 320g. According to the depth of contusion, it was divided into 1.2mm group (n = 12) and 2mm group (n = 12). Under anaesthesia, the left spinal cord of C5 rats was exposed, and the cervical spine was fixed by special clamps and attached to the stereotactic frame of the rat. Then the left side of the C5 spinal cord was injured with 1.5 mm diameter cylindrical head at 500 mm / s at 1.2 mm and 2 mm depth, respectively. The maximum impact force and contusion energy were monitored. The motor function scores of forelimbs, column climbing test and combing test were performed in each rat after injury. The materials were collected 8 weeks after injury. The area of spinal cord injury at the center of injury and gray were measured on the transverse section of spinal cord. Results: the maximum impact force and contusion energy of moderate injury group were 0.99 卤0.14 N and 1.08 卤0.01) 脳 10 ~ (-5) J, respectively. In severe injury group, 1.53 卤0.22N and 8.56 卤0.02) 脳 10 ~ (-5) J respectively. There was a statistical difference between the two groups in impact power and injury energy (P 0.05). No obvious neurological dysfunction was found in the control group after operation. The motor function score of forelimb was 16.29 卤0.49.The unilateral neurological dysfunction was found in all rats in the injury group, and 1.40 卤0.52 in the moderate injury group. There was no significant difference between the two groups in severe injury group (0.83 卤0.25). The forelimb motor function score of moderate injury group was 12.40 卤1.15 at 8 weeks after injury. The scores of severe injury group and control group were 8.41 卤0.83and 17.00 卤0.00respectively. The score of carding test was 3.80 卤0.60 in moderate injury group and 2.51 卤0.54 in severe injury group. The control group was 5.00 卤0.00.The proportion of injury to ipsilateral forelimb was 32.31 卤5.70 in moderate injury group and 4.92 卤1.21% in severe injury group. The control group was 49.42 卤1.20%, and there was statistical difference between the two groups (P 0.05). Under the microscope, the spinal cord tissue of the injured group showed obvious hemispheric cavity and structural damage. The contralateral spinal cord structure was basically intact. At the center of the injury, the dorsal spinal tract, the dorsal lateral tract and the posterior horn of the spinal cord were damaged in the moderate injury group, while in the severe injury group, only the ventrolateral tract of the remnant part of the spinal cord was found. The remaining areas of gray matter and white matter were 1.45 卤0.11mmm2 and 4.13 卤0.29mmm2 in moderate injury group, respectively. The severe injury group was 1.10 卤0.05mm-2 and 3.84 卤0.19mmm2, respectively. Conclusion: the model of cervical spinal cord contusion in different degree was established successfully in this study. Different contusion depth showed different degree of unilateral forelimb motor dysfunction and spinal cord tissue damage, which provided a basis for the study of cervical spinal cord injury model.
【作者单位】：南方医科大学南方医院脊柱骨科;
【基金】：国家自然科学基金(81601904) 广东省自然科学基金(2014A030313336)
【分类号】：R-332;R651.2
【正文快照】： 脊髓损伤(spinal cord injury,SCI)多由交通伤深度建立不同程度的大鼠C5脊髓半侧挫伤,事故、坠落和运动损伤引起,因椎体骨折撞击脊髓同时评价其损伤后的神经功能和组织形态改变,导致脊髓挫伤。近40%的脊髓损伤发生于颈脊髓为不同程度颈脊髓半侧挫伤动物模型的应用研究段[1],引

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