冲击波对人骨髓间充质干细胞增殖和成骨分化作用的研究

发布时间：2018-02-01 18:51

本文关键词： 冲击波骨髓间充质干细胞细胞增殖成骨分化　出处：《中国实验血液学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨冲击波对人骨髓间充质干细胞(MSC)增殖和成骨分化能力的影响。方法:从健康志愿者骨髓中分离培养骨髓间充质干细胞,将其分为3组,空白对照组细胞用普通培养液培养,成骨诱导组细胞用经典成骨诱导剂培养,冲击波组细胞用普通培养液培养,外加冲击波刺激。刺激后在镜下观察MSC形态;应用CCK-8检测冲击波组细胞和空白对照组MSC增殖能力的变化;第14天和28天分别用碱性磷酸酶和Von Kossa染色检测各组细胞的成骨能力。结果:CCK-8检测发现,冲击波与空白对照组相比,细胞的增殖活性显著增强;碱性磷酸酶和Von Kossa染色结果表明,冲击波组细胞的成骨分化能力比成骨诱导组要高;空白对照组细胞染色呈现弱阳性。结论:冲击波能促进骨髓间充质干细胞的增殖能力和成骨分化能力。
[Abstract]:Objective: to investigate the effect of shock wave on the proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (MSCs). Methods: bone marrow mesenchymal stem cells were isolated from healthy volunteers and divided into 3 groups. The cells of blank control group were cultured in ordinary culture medium, the cells of osteoblast induction group were cultured with classical osteogenic inducer, and the cells of shock wave group were cultured in ordinary culture medium. The morphology of MSC was observed under microscope after shock wave stimulation. CCK-8 was used to detect the proliferation ability of MSC in shock wave group and blank control group. Alkaline phosphatase and Von Kossa staining were used to detect the osteogenic ability of the cells in each group on the 14th and 28th days. Results the shock wave was compared with the control group. The proliferative activity of the cells was significantly increased. The results of alkaline phosphatase and Von Kossa staining showed that the osteogenic differentiation ability of shock wave group was higher than that of osteoblast induction group. Conclusion: shock wave can promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.
【作者单位】：中国人民解放军总医院骨科运动医学中心;军事医学科学院基础医学研究所细胞生物学研究室;火箭军总医院骨科;青岛世纪杰创医疗科技有限公司;
【基金】：国家自然科学基金面上项目(81371945,81572159,31370916);
【分类号】：R329.2
【正文快照】： 骨髓间充质干细胞(mesenchymal stem cell,MSC)具有多向分化潜能,其在特定环境下可向骨,软骨和脂肪等多种组织分化,因此在组织修复与再生方面具有重要的应用价值[1’2]。但是如何有效地调节其增殖,诱导其定向分化一直是再生医学领域的研究热点。冲击波是一种机械性脉冲冲击波,

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