心脏Telocytes在年老大鼠心脏的分布

发布时间：2018-02-02 23:20

  本文关键词： 心脏 Telocyte 心肌 衰老 免疫荧光 大鼠　出处：《解剖学报》2016年06期 　论文类型：期刊论文

【摘要】：目的探讨心脏Telocytes(CTs)在年老大鼠心脏的形态、分布及与年青心脏比较的异同。方法使用透射电子显微镜、生物测量、免疫荧光染色等技术,对24月龄年老大鼠心脏的心房部分、心室中间部分及心尖部分的代表性横切面中,CTs的形态、分布与细胞密度进行半定量分析。结果年老心脏3个代表性横切面的心肌细胞间隙和微血管周边均存在许多CTs,上述3个不同部位的CTs的形态指标的半定量分析值十分接近,差异没有统计学意义。经分离的年老心脏CTs与年青心脏一样表达c-Kit和CD34。对3个代表性横切面CTs细胞密度的半定量分析结果显示,年老大鼠心房部分的CTs密度[(47.65±4.01)个/mm~2]显著高于心尖部分[(33.68±2.53)个/mm2]和中间部分[(26.49±2.11)个/mm~2](P0.05)。年老心脏CTs在上述区域分布的差异,与我们前期所观察到的年青大鼠心脏相应区域CTs的分布规律相一致[7],但年老心脏上述3个区域CTs的密度均显著性高于年青心脏对应区域(P0.05)。结论年老心脏存在CTs,其心房部分的CTs密度显著高于心尖部分和心室中间部分,该分布规律与年青大鼠相一致,但年老心脏上述区域CTs的密度显著高于年青心脏。
[Abstract]:Objective to investigate the morphology, distribution and comparison of cardiac Telocytes-CTs in aged rats. Methods Transmission electron microscopy (TEM) and biological measurement were used. The morphology of CTs in the typical transverse section of the heart of 24 month old rats was studied by immunofluorescence staining. The central part of the heart and the middle part of the ventricle and the apical part of the heart. Results there was a lot of CTs in the intercellular space and the periphery of microvessel in the three representative cross-sectional sections of the aged heart. The semi-quantitative analysis values of the morphological indexes of the above three different parts of CTs were very close to each other. The difference was not statistically significant. The isolated aged heart CTs expressed c-Kit and CD34 the same as the young heart. The semi-quantitative analysis of the density of CTs cells in the three representative transverse sections showed. CTs density in the atrium of aged rats. [47.65 卤4.01) / mmmmm2] was significantly higher than the apical part. [33.68 卤2.53% / mm2] and middle part. [The distribution of CTs in the aged heart was different in the above regions. This is consistent with the distribution of CTs in the corresponding region of the heart of the young rat. [However, the density of CTs in the above three regions of the aged heart was significantly higher than that in the young heart (P 0.05). Conclusion there is CTs in the aged heart. The density of CTs in the atrial part was significantly higher than that in the apical part and the middle part of the ventricle, which was consistent with that of the young rat, but the density of CTs in these areas of the aged heart was significantly higher than that in the young heart.
【作者单位】： 暨南大学再生医学教育部重点实验室;暨南大学科技部及广东省国际科技合作基地;暨南大学发育与再生生物学系;
【基金】：国家高技术研究发展863计划(2007AA02Z105) 国家自然科学基金(30973158,81170324,81470433) 科技部港澳台合作专项(2012DFH30060) 广东省教育厅国际合作平台项目(2012gjhz0003) 广东省自然科学基金重点项目(04105826,S2012020010895) 广东省科技厅国际合作平台项目(2009B050900007,2013B051000062) 广东省科技计划项目(2015B020211010)
【分类号】：R339.3
【正文快照】： 心脏Telocytes细胞(CTs)是存在于心肌层,胞体较小,胞质和胞核比接近1比1,且胞体具有极长突起的一类新型心肌层间质细胞[1~3]。超微形态结构结果提示其可能是心肌层结构微环境的重要构成细胞之一,在心肌层生理功能的维持和损伤心肌的修复与再生中充当重要的调控作用[4~6]。我们，

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