针刺对干眼模型兔胆碱能神经元ChAT表达的影响

发布时间：2018-02-05 04:43

本文关键词： 针刺干眼胆碱乙酰转移酶胆碱能神经元　出处：《辽宁中医杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:通过观察针刺对干眼模型兔泪液分泌量及各脑区神经元中Ch AT含量的变化,探讨针刺治疗干眼模型兔的作用机制。方法:新西兰兔随机分为正常组、模型组、西药组和针刺组4组,每组4只。正常组不做任何处理;模型组皮下注射氢溴酸东莨菪碱,2.0 mg/次,4次/d,直至实验结束;西药组和针刺组从干眼动物模型制作成功开始,分别滴1%毛果芸香碱滴眼液和针刺治疗,连续治疗7 d。采用Schirmer试纸检测各组实验兔不同时间段泪液分泌量,采用免疫荧光组织化学方法,观察各组实验兔各脑区神经元中Ch AT表达的变化。结果:Schirmer试纸检测泪流量结果显示,在实验第28天即治疗7 d后,针刺组与西药组与实验第21天相比,泪流量明显增加;实验第28天,针刺组与西药组分别与模型组比较,差异具有显著性(P0.05)。Ch AT免疫阳性神经元表达与分布显示,Ch AT免疫反应阳性产物较多位于脑桥的被盖背侧核和臂旁外侧核内。其阳性物质计数显示,西药组、针刺组分别与模型组比较,被盖背侧核和臂旁外侧核中Ch AT免疫阳性神经元表达个数均增多,且均值具有显著差异性(P0.05)。平均光密度值显示,西药组、针刺组分别与模型组比较,以上两核团内Ch AT免疫阳性细胞平均光密度值均大于模型组。结论:毛果芸香碱滴眼液和针刺治疗均可增加脑桥的被盖背侧核和臂旁外侧核的Ch AT表达,促进兔泪腺分泌泪液,从而改善干眼症状。
[Abstract]:Objective: to observe the changes of tear secretion and the content of chat in neurons of dry eye model rabbits after acupuncture. Methods: new Zealand rabbits were randomly divided into 4 groups: normal group, model group, western medicine group and acupuncture group. In the model group, scopolamine hydrobromide was injected subcutaneously (2.0 mg / d) for 4 times / d until the end of the experiment. The western medicine group and the acupuncture group started successfully from the dry eye animal model, dripping 1% pilocarpine eye drops and acupuncture treatment respectively. After 7 days of continuous treatment, the tear secretion of each group was detected with Schirmer test paper and immunofluorescence histochemical method was used. The changes of chat expression in the brain neurons of each group were observed. Results the tear flow was detected on the 10% Schirmer test paper, which was 7 days after treatment on the 28th day of the experiment. Compared with the western medicine group, the lacrimal flow in the acupuncture group was significantly higher than that in the western medicine group on the 21st day. On the 28th day of experiment, the expression and distribution of immunoreactive neurons in acupuncture group and western medicine group were significantly different from those in model group. Chat immunoreactive products were located in dorsal tegmental nucleus and lateral parabrachial nucleus of the pontine. The positive substance count showed that the western medicine group and acupuncture group were compared with the model group. The number of chat immunoreactive neurons in dorsal tegmental nucleus and lateral parabrachial nucleus increased, and the mean value was significantly different (P 0.05). The acupuncture group was compared with the model group. The average optical density of chat immunoreactive cells in the above two nuclei was higher than that in the model group. Conclusion: both pilocarpine eye drops and acupuncture can increase Ch in dorsal tegmental nucleus of pontine and lateral parabrachial nucleus. AT expression. Promote tear secretion of rabbit lacrimal gland, thus improve dry eye shape.
【作者单位】：南京中医药大学;
【基金】：国家自然科学基金(81373746) 江苏省自然科学基金(BK2012856)
【分类号】：R245;R-332
【正文快照】： 干眼是最常见的眼表疾病之一,有关干眼的相关研究是近年来眼科领域研究的热点[1]。众多文献报道显示针刺治疗干眼疗效显著[2-5],临床应用安全,且针刺治疗干眼无论是即刻效应还是持续效应均优于人工泪液的治疗,针刺是治疗干眼的一条新途径[6]。针刺治疗干眼的实验研究也取得了

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