COPD伴抑郁障碍小鼠模型的建立及加味温胆汤的干预作用

发布时间：2018-02-05 21:47

本文关键词： 慢性阻塞性肺疾病抑郁烟熏小鼠加味温胆汤　出处：《南京中医药大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的建立一种烟熏诱导的小鼠慢性阻塞性肺疾病(COPD)伴抑郁障碍模型,并评价加味温胆汤对COPD伴抑郁障碍小鼠的治疗作用及其机制。方法将小鼠随机分为正常组与模型组,模型组每天烟熏4次。烟熏满15周后,测试正常组与模型组糖水消耗,观察二者气道和肺组织的病理改变等,在确认COPD伴抑郁障碍小鼠模型成功后,将模型组小鼠随机分为模型组、加味温胆汤高剂量组、加味温胆汤低剂量组及阳性药组,连续给药3周,给药结束后,测试各组小鼠的糖水消耗、悬尾不动时间,检测肺功能,并检测小鼠支气管肺泡灌洗液(BALF)中炎症细胞数,观察气道和肺组织病理改变等。结果与正常组相比,模型组小鼠在烟熏满15周时,体质量下降,糖水消耗比例降低,增强呼吸间歇(Penh)升高。连续给药3周后,与模型组相比,加味温胆汤组小鼠体质量增加,糖水消耗升高,悬尾不动时间减少,说明其抑郁样症状减轻;显著降低模型组小鼠吸气阻力(RI),升高肺动态顺应性(Cdyn);显著降低总白细胞数与中性粒细胞数;降低肺泡灌洗液以及海马组织中炎症因子等。结论用烟熏法所建立的COPD伴抑郁障碍小鼠模型较为符合本病的病程发展及临床特征,中药复方加味温胆汤对COPD伴抑郁障碍小鼠具有疗效,能够改善其病理与功能的改变,其机制可能与降低外周及中枢炎症有关。
[Abstract]:Objective to establish a smoke-induced model of chronic obstructive pulmonary disease (COPD) with depression in mice. Methods the mice were randomly divided into normal group and model group, the model group was smoked 4 times a day, and 15 weeks after smoking. The consumption of sugar water was measured in normal group and model group, and the pathological changes of airway and lung tissue were observed. After confirming the success of COPD with depressive disorder mice, the model group mice were randomly divided into model group. High dose group, low dose group and positive drug group of Jiawei Wendan decoction, continuously administered for 3 weeks. After administration, the consumption of sugar water, suspension time and lung function were measured in each group. The number of inflammatory cells in the bronchoalveolar lavage fluid (BALF) and the pathological changes of the airway and lung tissue were observed. Results compared with the normal group, the body mass of the model group decreased after 15 weeks of fumigation. After 3 weeks of continuous administration, compared with the model group, compared with the model group, compared with the model group, compared with the model group, the body weight of the mice increased, the consumption of sugar water increased, and the time of tail suspension decreased. The depression-like symptoms were alleviated. The inspiratory resistance of the model group was significantly decreased, and the pulmonary dynamic compliance was increased. The total leukocyte count and neutrophil count were significantly decreased. Conclusion the model of COPD with depressive disorder established by fumigation accords with the course and clinical characteristics of the disease. Chinese herbal compound Jiawei Wendan decoction has curative effect on COPD mice with depressive disorder and can improve its pathological and functional changes. The mechanism may be related to the reduction of peripheral and central inflammation.
【作者单位】：南京中医药大学中医脑病重点实验室;南京中医药大学附属中西医结合医院;
【基金】：国家自然科学基金(81403041,81673625) 江苏省自然科学基金(BK20140961,BK20151568)
【分类号】：R285.5;R-332

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