猬迭宫绦虫裂头蚴感染小鼠皮下肌肉组织β1转化生长因子含量变化的观察

发布时间：2018-02-08 10:22

本文关键词： 猬迭宫绦虫裂头蚴 β转化生长因子 HE染色免疫组织化学　出处：《中国寄生虫学与寄生虫病杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨β1转化生长因子(TGF-β1)在猬迭宫绦虫(Spirometra erinacei)裂头蚴感染小鼠皮下肌肉组中的表达情况及意义。方法对采自野生王锦蛇(Elaphe carinata)的裂头蚴进行形态学观察和PCR检测。昆明小鼠80只,用数字随机表法挑选40只,雌雄各半作为实验组,剩余40只为对照组。用蛇源裂头蚴经口喂饲实验组小鼠,5条/鼠。于喂饲后第7、14、28、56天各随机剖杀10只,分别收集含有裂头蚴寄生的皮下肌肉组织,用中性甲醛固定,制作石蜡切片。HE染色后,观察裂头蚴感染病灶周围的病理变化和纤维化程度;免疫组化检测皮下肌肉中TGF-β1的含量变化。对照组不喂饲裂头蚴,检测时间及方法同实验组。结果PCR扩增获得约400 bp的目的条带。测序结果显示,该裂头蚴COX1基因与Gen Bank中的猬迭宫绦虫序列一致性为99.12%。HE染色检查,皮下肌肉中的裂头蚴被炎性囊壁所包裹,虫体与囊壁之间形成穴腔,穴腔有时出现少许的浆液或血液。囊壁最里面的区域有薄层的纤维蛋白、坏死的碎片,壁中早期以中性粒细胞浸润为主,也有嗜酸粒细胞、巨噬细胞、浆细胞等,随着病程的进展,炎性囊壁逐渐扩大,壁中以慢性炎性细胞浸润为主,囊壁周围是宿主的组织细胞,纤维结缔组织增生明显,并有不同程度的纤维化。免疫组化检查结果显示,TGF-β1的主要表达部位为裂头蚴周围的炎症反应带和纤维结缔组织增生部位。TGF-β1相对表达量在感染后逐渐增高,于感染第28天达峰值(0.654 5±0.045 5),第56天时明显下降。在感染后第7~56天,实验组的TGF-β1水平为(0.502 6±0.008 2)~(0.346 8±0.030 4)与同期对照组的(0.270 0±0.001 6)~(0.274 0±0.005 1)比较,差异均有统计学意义(P0.05)。结论小鼠感染猬迭宫绦虫裂头蚴早、中期,TGF-β1表达水平均提高,其免疫抑制作用不利于清除和控制皮下肌肉组织中的裂头蚴。
[Abstract]:Objective to investigate the transforming growth factor beta 1 (TGF- beta 1) in Spirometra erinaceieuropaei (Spirometra erinacei) crack expression and the significance of subcutaneous muscle group in plerocercoid infection. Methods collected from wild Wang Jinshe (Elaphe carinata) the sparganum morphological observation and PCR detection. 80 Kunming mice, using digital randomly selected 40 rats, male and female as the experimental group, the remaining 40 rats as the control group. With the snake source sparganum oral feeding in the experimental group, 5 rats were fed to /. 7,14,28,56 days after the random killing of 10, were collected from muscle tissue containing sparganum parasitic skin next, fixed by formalin, paraffin sections after.HE staining, observe pathological changes and fibrosis degree of fissure lesions around plerocercoid infection; immunohistochemical changes of the content of TGF- beta 1 detection of subcutaneous muscle. The control group fed with sparganum, detection time and the results of PCR method with the experimental group. About 400 BP amplified purpose bands. The sequencing results showed that the sparganum COX1 gene and Gen Bank of Spirometra erinaceieuropaei sequence consistency for 99.12%.HE staining, subcutaneous sparganosis in muscle surrounded by inflammatory cystic wall, cavity formed between the body and the cystic wall, a little serous or blood cavity. The cyst wall has the innermost region of fibrin layer, necrotic debris, wall early by neutrophils, also have eosinophils, macrophages, plasma cells, with the progression of inflammatory cystic wall in chronic gradually expanded. Inflammatory cell infiltration, cystic wall surrounding host tissue, connective tissue hyperplasia, and there are different degrees of fibrosis. Immunohistochemical examination showed that TGF- mainly expressed in parts of the beta 1 inflammation sparganum surrounding connective tissue hyperplasia with fiber and parts .TGF- beta 1 expression increased gradually after infection, infection in twenty-eighth Tianda peak (0.6545 + 0.0455), the fifty-sixth day decreased significantly. At 7~56 days after infection, the experimental group TGF- beta 1 level (0.5026 + 0.0082) ~ (0.3468 + 0.0304) compared with the control group (0.2700 + 0.0016) ~ (0.2740 + 0.0051), the differences were statistically significant (P0.05). Conclusion the mice infected with sparganosis Spirometra erinaceieuropaei early, mid, TGF- beta 1 expression levels were increased, the immunosuppressive effect is not conducive to the removal and control of subcutaneous sparganosis in muscle tissue.

【作者单位】：贵州医科大学人体寄生虫学教研室;
【基金】：贵州省科技厅社会发展攻关项目(No.2014SY3024)~~
【分类号】：R383.35

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