流体剪切力对成骨样细胞BMP2和Smad4表达的实验研究

发布时间：2018-02-09 01:36

本文关键词： 流体剪切力 BMP2 Smad4 免疫荧光双标记信号转导　出处：《兰州大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨流体剪切力(FSS)对骨形态发生蛋白2(BMP2)通路中BMP2和Smad4表达的影响。方法：通过流体剪切力加载装置对体外培养成骨样细胞MG63施加12dyn/cm2的流体剪切力Omin,15min,30min,60min和120 min,应用免疫荧光双标记法检测不同时间流体剪切力作用于MG63细胞中BMP2及Smad4的表达。采用SPSS13.0软件包对数据进行单因素方差分析。结果：在12dyn/cm2的流体剪切力作用下,MG63成骨样细胞中BMP2及Smad4的表达明显增多(F=13.532,P=0.000; F=9.058, P=0.001),60min达高峰；所有组间BMP2表达有显著性差异(P0.05)；在Omin,15min,30min和120 min组,Smad4的表达有显著性差异(P0.05),在60 min组,Smad4的表达无显著性差异(P0.05)。结论：BMP2通路是流体剪切力信号转导的重要通路,适宜的流体剪切力刺激通过BMP2表达增强而引起Smad4在胞浆内积聚,结合于Smad复合物,转移至细胞核,诱导或抑制靶基因的转录。
[Abstract]:Objective: to investigate the effect of fluid shear stress (FSS) on the expression of BMP2 and Smad4 in bone morphogenetic protein 2 (BMP2) pathway. Immunofluorescence double labeling method was used to detect the expression of BMP2 and Smad4 in MG63 cells exposed to fluid shear stress at different time. The data were analyzed by single factor ANOVA using SPSS13.0 software package. Results: MG63 was formed by fluid shear stress of 12 dyn / cm ~ 2. The expression of BMP2 and Smad4 in osteoid cells increased significantly (P < 0.05), the expression of BMP2 and Smad4 increased significantly (P < 0.05), and the expression of BMP2 and Smad4 increased significantly (P < 0.05), and the expression of BMP2 and Smad4 increased significantly (P < 0.05). There was significant difference in the expression of BMP2 between all groups (P 0.05), in 30 minutes after 15 mins and 120 min, there was a significant difference in the expression of Smad4, but there was no significant difference in the expression of Smad4 in 60 min group. Conclusion the expression of BMP2 is an important pathway in signal transduction of fluid shear stress, and there is no significant difference in the expression of Smad4 in 60 min group. Appropriate fluid shear stress stimuli induce the accumulation of Smad4 in the cytoplasm through enhanced BMP2 expression, bind to the Smad complex, transfer to the nucleus and induce or inhibit the transcription of the target gene.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R312

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