海马细胞胞浆RARα表达对大鼠学习记忆功能的影响

发布时间：2018-02-09 19:09

本文关键词： RARα 胞浆表达海马细胞 GluR 学习记忆功能　出处：《第三军医大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨海马细胞胞浆视黄酸受体(retinoic acid receptor,RAR)α蛋白表达对大鼠空间学习记忆功能的影响。方法 SD大鼠采用完全随机化分组方法分为Morris水迷宫(Morris water maze,MWM)训练组(n=6)和未训练组(n=6),检测海马组织RARα和GluR1表达水平。分别将AAV-control sh RNA和AAV-RARαsh RNAs海马定位注射SD大鼠后,检测其学习记忆功能及海马组织RARα和GluR1表达水平变化。体外利用神经生长因子(neuron growth factor,NGF)诱导PC12细胞成熟分化,检测RARα和GluR1表达水平、胞内钙活性及静息膜电位。结果水迷宫训练组大鼠海马组织RARα和GluR1总蛋白和胞浆蛋白表达水平均较未训练组显著增加(P0.01)。海马定位注射2周后,Morris水迷宫检测发现AAV-RARαsh RNAs组大鼠学习记忆功能较对照组明显减弱(P0.05),同时海马RARα和GluR1总蛋白和胞浆蛋白表达水平也明显下降(P0.01)。经NGF诱导后的PC12细胞静息膜电位更接近于神经元静息膜电位(P0.01),RARα和GluR1总蛋白和胞浆蛋白表达水平较对照组明显增高(P0.01),胞内钙活性明显增强(P0.05)。结论海马细胞胞浆RARα蛋白表达与学习记忆能力呈正相关,在调节突触可塑性中发挥重要作用。
[Abstract]:Objective to investigate the effect of retinoic acid receptor RAR- 伪 protein expression in hippocampal cells on spatial learning and memory in rats. Methods Sprague-Dawley rats were randomly divided into two groups: Morris water maze and Morris water mazeM6) and untrained rats. The expression levels of RAR 伪 and GluR1 in hippocampus were detected in the training group. AAV-control sh RNA and AAV-RAR 伪 sh RNAs were injected into the hippocampus of SD rats. The learning and memory function and the expression level of RAR 伪 and GluR1 in hippocampal tissue were measured. In vitro, PC12 cells were induced to mature and differentiated by nerve growth factor neuron growth factor-NGF (NGF), and the expression levels of RAR 伪 and GluR1 were detected. Results the expression levels of RAR 伪 and GluR1 total protein and cytoplasmic protein in hippocampus of rats in water maze training group were significantly higher than those in control group (P 0.01). AAV-RAR 伪 sh was detected by Morris water maze test 2 weeks after hippocampal localization injection. The learning and memory function of RNAs group was significantly lower than that of control group, and the expression levels of RAR 伪, GluR1 total protein and cytoplasmic protein in hippocampus were also significantly decreased in RNAs group. The resting membrane potential of PC12 cells induced by NGF was closer to that of neuron resting membrane potential. Compared with the control group, the expression levels of total protein and cytoplasmic protein of Rar 伪 and GluR1 were significantly increased, and the activity of intracellular calcium increased significantly. Conclusion the expression of RAR 伪 protein in the cytoplasm of hippocampal cells is positively correlated with the ability of learning and memory. Plays an important role in regulating synaptic plasticity.
【作者单位】：重庆医科大学附属儿童医院营养研究中心儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地认知发育与学习记忆障碍转化医学重庆市重点实验室;
【基金】：国家自然科学基金-中加合作重点项目(81161120498);国家自然科学基金面上项目(81271385,81100476)~~
【分类号】：R338

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