跑台运动对PD模型大鼠运动皮层异常β振荡的调节作用

发布时间：2018-02-09 23:58

本文关键词： 跑台运动帕金森病运动皮层局部场电位 β振荡　出处：《西安体育学院学报》2017年05期 　论文类型：期刊论文

【摘要】：目的观察运动对帕金森病(parkinson's disease,PD)模型大鼠初级运动皮层(M1)异常β振荡的调节作用,探究运动干预改善PD行为功能的可能原因。方法清洁级SD大鼠18只分为3组,即假手术组(Control,n=5)、PD组(PD,n=6)和PD运动组(PD+Ex,n=7),于内侧前脑束注射6-羟基多巴(6-OHDA)同时于M1区植入多通道电极。大鼠在造模后第1周颈部皮下注射阿朴吗啡(APO)旋转行为实验评价模型成功;PD+Ex组在第1周开始进行跑台训练(11 m/min,30 min/d,5 d/周,4周),各组大鼠在第0/1/2/3/4采用自主错步仪进行行为测试;采用在体多通道电生理技术,观察各组大鼠M1区在第0/1/2/3/4的局部场电位(LFPs),免疫组化技术检测黑质和纹状体内酪氨酸羟化酶(TH)表达。结果 TH免疫组化结果显示,PD+Ex组较PD组无显著性改变(P0.05);自主错步行为测试结果发现,PD+Ex组的通过时间、前肢错步数和尾部滑落数及潜伏期较PD组均有显著性改善(P0.01)。电生理学结果显示,PD组较Control组10~30 Hz的β频段异常增高(P0.01);PD+Ex组在第1周β振荡的PSD较第0周无显著改变(P0.05),在第2/3/4周(P0.05)显著降低。结论运动干预调节运动皮层的功能活性,抑制M1区异常β振荡,进而改善其步态及自主活动能力。推测运动降低PD模型大鼠M1区异常β振荡介导了皮层-纹状体Glu能突触可塑性,调控输入至基底神经节的运动编码信息,最终改善大鼠的行为功能。
[Abstract]:Objective to observe the effect of exercise on 尾 oscillation in primary motor cortex (M1) of Parkinson's disease (PD) rats, and to explore the possible causes of exercise intervention to improve PD behavioral function. Methods 18 clean SD rats were divided into 3 groups. That is, the sham operation group (control group) and PD group (PD group) and PD exercise group (PD exann 7) were injected into the medial forebrain tract of 6-hydroxydopazone 6-OHDA.The rats were injected with multichannel electrodes in M1 region simultaneously. The rotation behavior of apomorphine apo was injected subcutaneously into the neck of rats at the first week after modeling. The model was successfully established in the PD Ex group at the beginning of the first week. The rats in each group were tested with an autonomous stagger at 0 / 1 / 2 / 3 / 4 by using an autonomous error-walker. The rats in each group were trained at 11 m / min ~ 30 min / d ~ (-1) min / d ~ (-1) / d ~ (5) d / week for 4 days / week. Using in vivo multichannel electrophysiology, The expression of tyrosine hydroxylase (THH) in substantia nigra and striatum was detected by immunohistochemistry. The wrong walk was used to find the passage time of the PD Ex group as a result of the test. The results of electrophysiology showed that the PSD of PD group was significantly higher than that of Control group at 10 ~ 30 Hz. The PSD of 尾 oscillation in PD group at the 1st week was not significantly different from that in the 0 ~ (th) week. P0.05 decreased significantly at the 2nd / 3rd to 4th week (P 0.05). Conclusion exercise intervention regulates the functional activity of motor cortex. The abnormal 尾 oscillation in M1 region was inhibited, and then its gait and autonomous activity were improved. It is speculated that the abnormal 尾 oscillation in M1 region of PD model rats mediated the synaptic plasticity of cortex-striatal Glu. The motor coding information inputted to the basal ganglia can improve the behavioral function of rats.
【作者单位】：北京师范大学体育与运动学院;
【基金】：国家自然科学基金项目(31571221) 北京市自然科学基金项目(5142012)
【分类号】：G804.2;R-332

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