大鼠白色和棕色脂肪来源的间充质干细胞成脂分化特性的比较

发布时间：2018-02-11 23:43

本文关键词： 解耦联蛋白棕色脂肪组织白色脂肪组织脂肪间充质干细胞成脂诱导免疫荧光大鼠　出处：《解剖学报》2016年05期 　论文类型：期刊论文

【摘要】：目的探讨白色脂肪组织(WAT)和棕色脂肪组织(BAT)来源的间充质干细胞成脂分化特性的差异。方法雄性SD大鼠15只,3只用于细胞分离培养,12只用于细胞移植。体外分离培养WAT和BAT两种来源的脂肪干细胞,用油红O染色检测两种干细胞的成脂分化率,用免疫荧光技术检测成脂诱导、分化后的细胞是棕色脂肪细胞还是白色脂肪细胞。4’6-二脒基-2-苯基吲哚(DAPI)标记两种来源的干细胞后移植至腹股沟区,分别在第1、2、3周取材,免疫荧光技术检测植入细胞的分化趋向。结果 WAT来源的干细胞增殖速度明显快于BAT来源的干细胞,前者成脂分化率为0.205±0.069,后者为0.165±0.053,两种干细胞的成脂诱导率差异无统计学意义(P0.05)。两种干细胞分别植入体内后,在第1、2、3周发现,两种干细胞均表达棕色脂肪特异性蛋白解耦联蛋白1(UCP1)。结论 WAT和BAT来源的干细胞在体外及体内诱导成脂后均分化为棕色脂肪细胞。
[Abstract]:Objective to investigate the difference of adipogenic differentiation between white adipose tissue (WATT) and brown adipose tissue (BAT). Methods Fifteen male SD rats were used in cell isolation culture and 12 were used for cell transplantation. To culture adipose stem cells from both WAT and BAT, Oil red O staining was used to detect the adipogenic differentiation rate of the two stem cells, and immunofluorescence technique was used to detect the adipogenic induction. The differentiated cells were brown adipocytes or white adipocytes. Results the proliferation rate of stem cells derived from WAT was significantly faster than that of stem cells derived from BAT. The rate of adipogenic differentiation was 0.205 卤0.069 in the former and 0.165 卤0.053 in the latter. There was no significant difference in adipogenic induction rate between the two kinds of stem cells. Both stem cells expressed brown adipose-specific protein uncoupling protein 1 (UCP1). Conclusion Stem cells derived from WAT and BAT are evenly divided into brown adipocytes after fat-forming in vitro and in vivo.
【作者单位】：新乡医学院河南省医用组织再生重点实验室;新乡医学院第三附属医院心内科;
【基金】：国家自然科学基金(81400237)
【分类号】：R329.2

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