复方大承气汤灌服加中药脐眼外敷对肠粘连大鼠模型腹膜粘连程度的影响

发布时间：2018-02-12 22:18

本文关键词： 中草药大鼠肠粘连模型 TGF-β_ TNF-α 粘连程度　出处：《广东医学》2017年14期 　论文类型：期刊论文

【摘要】：目的研究复方大承气汤灌服和芒硝、冰片、吴茱萸适量混合后于脐眼外敷预防大鼠模型术后肠粘连的作用及机制。方法雄性SD大鼠120只,随机分为对照组、模型组、医用几丁糖组、生长抑素组、中草药一组和中草药二组。除正常对照组外,其余各组大鼠均采用Ellis法制备肠粘连模型,造模后对照组和模型组正常喂养,其余各组分别给予相应药物:医用几丁糖组在造模关腹时局部涂抹几丁糖;生长抑素组给予皮下注射醋酸奥曲肽0.1μg/g,1次/12 h;中草药一组给予复方大承气汤煎液0.05 mL/g灌服,2次/d;中草药二组在一组用药的基础上,用芒硝、冰片、吴茱萸适量混合后外敷于脐眼。各组于造模术后第8天下腔静脉取血后处死,ELISA法测定血清肿瘤坏死因子-α(TNF-α)含量,肉眼观察肠粘连的程度并分级,采用免疫组化法测定腹膜粘连组织转化生长因子-β_1(TGF-β_1)表达。结果与模型组对比,医用几丁糖组、生长抑素组、中草药一组和中草药二组大鼠血清中TNF-α含量依次降低,局部粘连程度依次减轻,粘连组织中TGF-β_1表达依次减少,中草药一组和二组与模型组、医用几丁糖组、生长抑素组相比差异有统计学意义(P0.05),中草药二组和中草药一组之间相比差异有统计学意义(P0.05),医用几丁糖组和生长抑素组间差异无统计学意义(P0.05)。结论联合应用复方大承气汤灌服和芒硝、冰片、吴茱萸适量混合后于脐眼外敷能显著抑制纤维结缔组织的增生,减轻造模大鼠模型局部粘连程度。
[Abstract]:Objective to study the effect and mechanism of compound Dachengqi decoction (Dachengqi decoction) on preventing intestinal adhesion after umbilical eye application. Methods 120 male Sprague-Dawley rats were randomly divided into control group and model group. Except for the normal control group, the other groups of rats made intestinal adhesion model by Ellis method, the control group and the model group were fed normally after the establishment of the model. The other groups were given corresponding drugs: chitosan was applied locally in the medical chitosan group when the model was closed; The somatostatin group was subcutaneously injected with octreotide acetate 0.1 渭 g / g for 12 h; the Chinese herbal medicine group 1 was given the compound Dachengqi decoction 0.05 mL/g decoction for 2 times per day; the second group was treated with mirabilite and borneol on the basis of one group. Evodia rutaecarpa was mixed with appropriate amount and applied to the umbilical eye. The serum levels of TNF- 伪 TNF- 伪 were determined by Elisa after the 8th operation of the model, and the degree and grade of intestinal adhesion were observed with naked eyes. The expression of TGF- 尾 in peritoneal adhesion tissue was determined by immunohistochemical method. Results compared with the model group, the serum TNF- 伪 levels in the medical chitosan group, somatostatin group, Chinese herbal medicine group and Chinese herbal medicine group were decreased in turn. The degree of local adhesion was reduced in turn, the expression of TGF- 尾 1 in adhesive tissue was decreased in turn, the Chinese herbal medicine group 1 and 2 and the model group, the medical chitosan group, There were significant differences in somatostatin group (P 0.05), Chinese herbal medicine group (2 groups) and Chinese herbal medicine group (1 group). There was no significant difference between medical chitosan group and somatostatin group (P 0.05). Dachengqi decoction and mirabilite, The mixture of borneol and rutaecarpa could significantly inhibit the proliferation of fibrous connective tissue and reduce the degree of local adhesion of rat model.
【作者单位】：广州市正骨医院ICU科;
【分类号】：R285.5;R-332

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