酸枣仁皂苷A对糖尿病模型大鼠肾小球细胞凋亡的影响

发布时间：2018-02-14 21:25

本文关键词： 糖尿病肾病酸枣仁皂苷A 细胞凋亡内源性线粒体通路转化生长因子β_　出处：《中国药理学与毒理学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的研究酸枣仁皂苷A对糖尿病模型大鼠肾小球细胞凋亡的影响,并初步探讨其可能的机制。方法 SD大鼠ip给予链脲菌素100 mg·kg~(-1),空腹血糖16 mmol·L~(-1)显示造模成功。糖尿病模型大鼠ip给予酸枣仁皂苷A 10和20 mg·kg~(-1),共4周。酶联免疫检测法测定大鼠血液中糖化血红蛋白(GHb)的含量,过碘酸雪夫(PAS)染色观察肾小球组织形态,TUNEL法检测肾小球细胞凋亡,免疫组化法检测肾组织中Bcl-2和Bax蛋白表达,Western蛋白印迹法检测肾组织中活化的胱天蛋白酶3和活化的胱天蛋白酶9蛋白表达,实时荧光PCR检测肾组织中转化生长因子β_1 (TGF-β_1 )mRNA表达。结果与模型组相比,给予酸枣仁皂苷A10和20 mg·kg~(-1)处理的大鼠血液中GHb含量均显著下降(mmol·L~(-1):10.9±0.8 vs 17.5±1.5,P0.05;7.6±0.5 vs 17.5±1.5,P0.05),肾小球的PAS阳性得分均明显降低(26.8±3.2 vs 36.4±3.8,P0.05;18.4±2.1 vs 36.4±3.8,P0.05),肾小球细胞凋亡均减少[(8.2±0.8)%vs(17.6±1.8)%,P0.05;(5.1±0.5)%vs(17.6±1.8)%,P0.05],肾组织中Bcl-2蛋白表达均显著增加(P0.05),而Bax(P0.05)、活化的胱天蛋白酶9(P0.05)和活化的胱天蛋白酶3(P0.05)蛋白表达均显著降低,肾组织中TGF-β_1 mRNA明显降低(P0.05)。结论酸枣仁皂苷A可以抑制糖尿病模型大鼠肾小球细胞凋亡,可能与内源性线粒体通路和TGF-β_1 mRNA的表达有关。
[Abstract]:Objective to study the effect of jujuboside A on glomerular cell apoptosis in diabetic rats. Methods Sprague-Dawley rats were given streptozotocin 100mg 路kg ~ (-1) and fasting blood glucose 16 mmol 路L ~ (-1). Diabetic rats were treated with jujube saponins A _ 10 and 20 mg 路kg ~ (-1) for 4 weeks by enzyme-linked immunosorbent assay (Elisa). Method to determine the content of glycosylated hemoglobin (GHB) in blood of rats. Apoptosis of glomerular cells was detected by Tunel method. The expression of Bcl-2 and Bax protein in renal tissue was detected by immunohistochemistry and the expression of activated cystatin 3 and activated cystatin 9 protein in renal tissue was detected by Western blot. The expression of TGF- 尾 1 mRNA in renal tissue was detected by real-time fluorescence PCR. The GHb content in blood of rats treated with jujube seed saponins A10 and 20 mg 路kg ~ (-1) significantly decreased significantly (10.9 卤0.8) vs 17.5 卤1.5 (P 0.057.6 卤0.5) vs 17.5 卤1.5 (P) 0.057.6 卤0.5 vs 17.5 卤1.5 (P 0.05). The positive scores of PAS in glomeruli were significantly decreased by 26.8 卤3.2 vs 36.4 卤3.8P0.0518.4 卤2.1 vs 36.4 卤3.8V / P5, and apoptosis was decreased [8.2 卤0.8V vs 17.6 卤1.8VS 17.6 卤1.8vs17.6 卤1.80.75], and the expression of Bcl-2 protein in renal tissue was also decreased. The expression of P0. 05), activated cystatin 9 (P0. 05) and activated cystatin 3 (P0. 05) were significantly decreased in Baxon P0. 05 (P 0. 05) and activated cystatin 3 (3) P0. 05). Conclusion jujuboside A can inhibit glomerular cell apoptosis in diabetic rats, which may be related to endogenous mitochondrial pathway and expression of TGF- 尾 1 mRNA.
【作者单位】：山西省长治医学院附属和济医院内分泌科;
【基金】：山西高等学校科技研究开发项目基金(3012430)~~
【分类号】：R285.5;R-332

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