肠道白色念珠菌感染对脾虚小鼠小肠黏膜中T细胞亚群和穿孔素-颗粒酶B的影响

发布时间：2018-02-20 17:30

  本文关键词： 脾虚证 白色念珠菌 T细胞亚群 穿孔素 颗粒酶B　出处：《中华中医药学刊》2017年08期 　论文类型：期刊论文

【摘要】：目的:通过观察肠道白色念珠菌感染对脾虚小鼠小肠黏膜固有层中T细胞亚群和穿孔素、颗粒酶的影响,探讨机体在脾虚状态下感染白色念珠菌的部分免疫机制。方法:本实验采用饮食失节加劳倦过度的复合因素制备小鼠脾虚模型,经肠道感染白色念珠菌后第14天,应用流式细胞术检测小鼠小肠黏膜固有层中CD_3~+、CD_4~+和CD_8~+T的细胞百分比,采用免疫荧光法检测各组小鼠小肠黏膜中穿孔素、颗粒酶阳性表达细胞数。结果:与正常对照组比较,其他3组小鼠小肠黏膜固有层中CD_4~+T细胞比例均有不同程度下降,CD_8~+T细胞比例均有不同程度上升,有统计学意义(P0.01);正常感染白念组和脾虚感染白念组小鼠小肠黏膜固有层中穿孔素阳性表达细胞均明显增多(P0.05或P0.01),而颗粒酶阳性表达细胞仅脾虚感染白念组明显增多(P0.01)。其中,脾虚感染白念组最显著,与其他3组均具有统计学意义(P0.05或P0.01)。结论:小肠黏膜固有层中穿孔素和颗粒酶的高表达可能是造成小肠局部损伤的免疫机制之一,脾虚小鼠肠道局部免疫功能下降,感染发生后可加重肠道局部损伤程度。
[Abstract]:Objective: to observe the effect of Candida albicans infection on T cell subsets, perforin and granzyme in lamina propria of spleen deficiency mice. To explore the partial immune mechanism of Candida albicans infection in spleen deficiency. Methods: the spleen deficiency model of mice was established by the compound factors of diet loss and overwork. The mice were infected with Candida albicans on the 14th day after intestinal infection. Flow cytometry was used to detect the percentage of CD3 ~ + CD4 ~ and CD8 ~ T in the lamina propria of small intestine of mice, and immunofluorescence assay was used to detect the number of perforin and granzyme positive cells in the intestinal mucosa of mice. The percentage of CD4 ~ T cells in the lamina propria of the other three groups decreased in varying degrees, and the percentage of CD8 ~ T cells increased in different degrees. The positive expression of perforin in the lamina propria of small intestine of normal infection group and spleen deficiency group were significantly increased (P 0.05 or P 0.01), while the expression of granzyme positive cells was significantly increased only in the group of spleen deficiency infection (P 0.01), and the expression of perforin in the lamina propria of small intestine of mice infected with spleen deficiency was significantly higher than that in group B (P 0.01). Conclusion: the high expression of perforin and granzyme in the lamina propria of small intestine may be one of the immune mechanisms of local injury of small intestine. The local immune function of spleen deficiency mice decreased, and the degree of local intestinal injury was aggravated after infection.
【作者单位】： 辽宁中医药大学;
【基金】：国家自然科学基金项目(81173145,81403208) 辽宁中医药大学省部共建中医脏象理论及应用教育部重点实验室项目
【分类号】：R259;R-332
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本文编号：1519572