糖尿病大血管病变“气阴两虚”态模型的建立

发布时间：2018-02-21 22:49

本文关键词： 糖尿病大血管病变气阴两虚动物模型　出处：《中国中医基础医学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:建立糖尿病大血管病变"气阴两虚"态模型。方法:60只雄性GK大鼠(随机血糖11.1 mmol·L~(-1)),按随机数字表法分为GK组、模型组、中药组各20只,另设正常Wistar大鼠20只为正常组。正常组、GK组喂饲普通饲料,模型组和中药组喂饲高脂饲料,造模同时中药组给予益气养阴中药治疗。5周后检测各组大鼠糖脂代谢、胰岛素抵抗指数(HOMA-IR)、血管内皮细胞凋亡和Caspase-3表达等指标,光镜下观测大血管组织病理。结果:与正常组和GK组比较,模型组大鼠血糖、TC和TG、胰岛素抵抗、大血管内皮细胞凋亡和腹主动脉病理比较差异有统计学意义,与模型组比较中药组所有指标均有显著改善。结论:通过GK大鼠喂养高脂饲料的方法可制备糖尿病大血管病变"气阴两虚"态动物模型。
[Abstract]:Objective: to establish a model of "deficiency of qi and yin" in diabetic macroangiopathy. Methods: sixty male GK rats were randomly divided into GK group, model group and traditional Chinese medicine group. In addition, 20 normal Wistar rats were given normal diet, the model group and traditional Chinese medicine group were fed with high fat diet. At the same time, the glucose and lipid metabolism of rats in each group were detected after the treatment of supplementing qi and nourishing yin Chinese medicine for 5 weeks. Insulin resistance index (HOMA-IRI), vascular endothelial cell apoptosis and Caspase-3 expression were measured under light microscope. Results: compared with normal group and GK group, blood glucose, TC, TGand insulin resistance in model group were measured. There were significant differences between the apoptosis of endothelial cells and the pathological changes of abdominal aorta. Conclusion: the animal model of "deficiency of Qi and Yin" in diabetic macrovascular disease can be established by feeding high fat diet to GK rats.
【作者单位】：成都中医药大学临床医学院;成都中医药大学附属医院;
【基金】：国家自然科学基金资助项目(81273749)-养阴益气活血法阻断糖尿病大血管病变GK大鼠代谢记忆的量效关系研究及表观遗传学机制;国家自然科学基金青年基金项目(81102589)-养阴益气活血法双向调控PI3-K信号系统对防治糖尿病大血管病变的拆方研究
【分类号】：R259;R-332

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