坤泰胶囊对VCD所致POI模型大鼠的疗效探究

发布时间：2018-02-24 06:21

本文关键词： 坤泰胶囊卵巢功能不全(POI) 去氧乙烯基环乙烯(VCD)　出处：《中成药》2017年04期 　论文类型：期刊论文

【摘要】：目的探究坤泰胶囊(黄连、黄芩、阿胶,等)对去氧乙烯基环乙烯(VCD)所致卵巢功能不全(POI)模型大鼠的疗效。方法 75只21日龄离乳大鼠,随机均分为佐剂对照组,模型组,坤泰胶囊低、中、高剂量组。除佐剂对照组腹腔注射芝麻油外,其余4组均给予腹腔注射VCD溶液。灌胃给药45 d。每组选取8只持续处于动情间期或持续动情周期紊乱的大鼠,进行检测大鼠血清促卵泡生成素(FSH)、促黄体生成素(LH)、雌二醇(E_2)、抗苗勒管激素(AMH);剖腹取出卵巢、子宫称重并进行形态学观察。结果与佐剂对照组相比,模型组大鼠血清FSH明显增高且AMH、E_2显著降低,LH有所增高;经坤泰中、高剂量干预后,FSH水平显著降低,AMH水平明显增加;模型组大鼠卵巢湿重较佐剂对照组减轻,坤泰胶囊干预后,低剂量组卵巢湿重增加明显;模型组大鼠各级卵泡及黄体计数均明显减少,坤泰胶囊干预后,各级卵泡计数明显增加,黄体计数有所增加;光镜下,模型组子宫内膜变薄,管壁子宫腺体数量明显减少。经坤泰胶囊干预后,内膜有所增厚,腺体数量明显增加。结论坤泰胶囊可以减缓模型大鼠卵巢功能衰减的速度。
[Abstract]:Objective to investigate the effect of Kun Tai capsule (Coptis chinensis, Radix Scutellariae, Gelatin, et al.) on ovarian dysfunction induced by deoxyvinyl cycloethylene (VCD) in rats. Methods Seventy-five 21-day-old isolated rats were randomly divided into adjuvant control group and model group. Except adjuvant control group, the other four groups were given intraperitoneal injection of VCD solution. Each group was given intraperitoneal injection of VCD solution for 45 days. Eight rats in each group were continuously in the period of estrus or continuous estrous cycle disorder. The serum follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E _ 2), anti-mullerian hormone (AMHN) were detected in rat serum, ovary was taken out by laparotomy, uterus was weighed and morphological observation was made. Results compared with adjuvant control group, In the model group, the serum FSH level was significantly increased and AMHH E2 was significantly decreased, while the serum FSH level was significantly decreased after high dose intervention, the wet weight of ovary in the model group was less than that in the adjuvant control group, and after the intervention of Kun Tai capsule, the rats in the model group were significantly lower than those in the adjuvant control group, and the rats in the model group were treated with Kuntai capsule. The number of follicles and corpus luteum in model group decreased obviously after intervention of Kun Tai capsule, the count of follicle and corpus luteum was increased, and the endometrium of model group was thinned under light microscope, the wet weight of ovary was increased obviously in low dose group, and the count of follicle and corpus luteum in model group decreased obviously after intervention of Kun Tai capsule. The number of uterine glands in the tube wall decreased obviously. After the intervention of Kuntai capsule, the endometrium was thicker and the number of glands was obviously increased. Conclusion Kun Tai capsule can slow down the decline of ovarian function in the model rats.
【作者单位】：南京中医药大学;浙江省中医院;
【基金】：江苏省科技计划项目临床医学科技专项(BL2013039) 横向课题,和颜坤泰胶囊对VCD所致大鼠卵巢功能损伤动物模型的保护作用研究(2015026)
【分类号】：R285.5;R-332

【相似文献】