小鼠Lewis肺癌不同部位皮下移植瘤模型的比较

发布时间：2018-02-24 12:38

本文关键词： 肺癌皮下移植瘤动物模型　出处：《中国实验动物学报》2017年04期 　论文类型：期刊论文

【摘要】：目的探究不同部位肺癌皮下移植瘤存在的差异,为肺癌研究者提供提供基础依据。方法将稳定表达虫萤光素酶的小鼠Lewis肺腺癌细胞(ll2-luc-m38)分别注射于C57 BL/6小鼠的右腋皮下、右腹股沟皮下、脚垫皮下,定期利用小动物活体成像系统观察小鼠皮下移植瘤成瘤情况及转移情况,观察不同部位荷瘤小鼠的生存时间和死亡率,肿瘤组织取材,采用石蜡包埋、切片、HE染色做出病理学诊断。并将小鼠处死后立即进行肺部组织固定、观察转移灶。对皮下移植瘤行切除术观察小鼠术后生存情况和转移情况。结果腋下组和腹股沟组成瘤时间较早,成瘤率为100%,脚垫组成瘤时间较晚,成瘤率为33%;腹股沟组和腋下组瘤体积增长迅速,其中腹股沟组瘤体积增长最快;接种后第21天腋下组70%的小鼠出现了肺转移,转移灶数目较多;腹股沟组50%小鼠出现肺转移,且转移灶数目较少;脚垫组小鼠未观察到肺转移灶。脚垫组小鼠死亡率最高。腹股沟组和腋下组小鼠可行皮下移植瘤切除术,术后存活率为100%。结论小鼠lewis肺癌皮下移植瘤模型中,腹股沟组和腋下组成瘤率高,可耐受移植瘤切除术,手术死亡风险低,便于监测,操作简单且可重复性高,其中腋下组转移性更好。
[Abstract]:To explore the differences exist in different parts of the lung cancer transplanted subcutaneously to provide the basis for the study of lung cancer. Methods the stable expression of luciferase in mice Lewis lung adenocarcinoma cells (ll2-luc-m38) were injected into C57 BL/6 mice subcutaneously in the right axilla and right inguinal subcutaneous, subcutaneous pad, regular mice transplanted subcutaneously into the condition of tumor and metastasis with small animal imaging system, observe the survival time and mortality in different parts of the tumor bearing mice, tumor tissue samples, using paraffin section, HE staining, and make pathological diagnosis. The mice were sacrificed immediately after lung tissue fixation, metastasis and metastasis. To observe the survival of skin tumor resection were observed after operation. Results the armpit and groin group consisting of tumor earlier, tumor formation rate was 100%, the Ottomans oncogenous late time, tumor was 33%; inguinal Group and lat group tumor volume increased rapidly, which is the fastest growing group of groin tumor volume; twenty-first days after inoculation of axillary group 70% mice developed lung metastasis, metastasis number; group 50% mice inguinal metastasis occurred, and the number of metastases is less; the Ottomans were not observed in mice lung metastasis. The highest mat group the mortality of mice. Mice were viable group and inguinal axillary subcutaneous transplantation tumor resection, postoperative survival rate was 100%. conclusion the mouse Lewis lung cancer xenograft model, inguinal and axillary group tumor formation rate is high, can withstand tumor excision surgery, the risk of death is low, easy monitoring, simple operation and high repeatability among them, axillary metastatic group better.

【作者单位】：北京中医药大学;首都医科大学附属北京中医医院;
【基金】：国家自然科学基金项目(编号:81473643) 首都医科大学基础-临床科研合作基金项目(编号:15JL79) 北京市医院管理局青苗计划项目(编号:QML20150903)
【分类号】：R-332;R734.2

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