钙敏感受体在大鼠轴型皮瓣缺血再灌注损伤模型中的表达和意义

发布时间：2018-02-24 14:42

本文关键词： 钙敏感受体缺血再灌注损伤轴型皮瓣　出处：《重庆医科大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究钙敏感受体(calcium-sensing receptor,Ca SR)在轴型皮瓣血管内皮细胞的表达,并探讨Ca SR在皮瓣缺血再灌注损伤过程中的重要作用。方法:构建以腹壁下浅动、静脉为蒂的大鼠轴型皮瓣,使用微动脉夹夹闭血管3 h建立轴型皮瓣缺血再灌注(ischemia/reperfusion,I/R)损伤模型。随后大鼠被随机分为3组(每组10只),分别通过股静脉给予Ca Cl2、NPS2143+Ca Cl2和生理盐水(normal saline,NS)治疗。再灌注2 h和7 d后,采用计算机辅助面积测量法评估皮瓣坏死;HE染色观察皮瓣血管形态学变化;TUNEL染色检测细胞凋亡;RT-PCR、免疫组化法分别检测血管内皮细胞中Ca SR、Cleaved Casepase-3、Bax和Bcl-2的表达情况。结果:与NS对照组和NPS2143+Ca Cl2组相比,Ca Cl2组皮瓣坏死率增多的同时[(14.87±1.02)%,P=0.000],TUNEL染色阳性细胞增多(2 h:46.960±1.965,7 d:48.855±3.550,P=0.000),Ca SR(2 h:0.157±0.011,7 d:0.129±0.015,PIHC=0.000;PPR-PCR=0.000)、Cleaved Casepase-3(2 h:0.101±0.020,7 d:0.098±0.011,PNS=0.046,PNPS2143+Ca Cl2=0.000)表达升高,并且Bax存在明显棕黄色染色,而Bcl-2几乎无表达。但是NPS2143+Ca Cl2组与NS对照比相比,结果刚好相反。结论:Ca SR在大鼠轴型皮瓣的血管内皮细胞中具有功能性表达,其表达水平与轴型皮瓣I/R损伤程度密切相关,并且抑制Ca SR表达能减轻轴型皮瓣I/R损伤。
[Abstract]:Objective: to study the expression of calcium-sensing receptor Ca SRR in vascular endothelial cells of axial flap and to explore the important role of Ca2 SR in the process of ischemia reperfusion injury. Methods: a rat axial flap pedicled with superficial inferior abdominal artery and vein was constructed. The model of ischemia reperfusion I / R injury of axial flaps was established with arteriole clamping for 3 h. Then the rats were randomly divided into 3 groups (10 rats in each group were treated with CaCl _ 2N _ 2NPS _ 2143 Ca Cl2 and normal saline normal saline group) respectively. After 2 hours and 7 days of perfusion, The changes of vascular morphology of the flap were evaluated by computer aided area measurement. Tunel staining was used to detect apoptosis RT-PCR, and immunohistochemical method was used to detect the expression of Ca SR-Cleaved Casepase-3Bax and Bcl-2 in vascular endothelial cells. Fruit: compared with NS control group and NPS2143 Ca Cl2 group, the necrosis rate of flap was increased in Ca Cl2 group [14.87 卤1.02P0.000] the expression of Tunel staining positive cells increased at 2 h: 46.960 卤1.965U 7 d, 48.855 卤3.550P0.000 Ca SR(2 0.157 卤0.0117d: 0.129 卤0.015PIHC0.000PPR-R0000 Casepase-3(2 Casepase-3(2 0.000 Casepase-3(2 cell 0.101 卤0.0207d 0.098 卤0.011PNS0.046PNPS2143Ca Cl20.000). There was obvious brown staining in Bax, but almost no expression of Bcl-2 in NPS2143 Ca Cl2 group, but the results were just the opposite compared with those in NS control group. Conclusion [WT5 "HZ] Ca SR has functional expression in vascular endothelial cells of rat axial flap. The expression level was closely related to the degree of I / R injury in axial flaps, and inhibition of Ca SR expression could alleviate I / R injury of axial flaps.
【作者单位】：重庆医科大学附属口腔医院口腔疾病与生物医学重庆市重点实验室重庆市高校市级口腔生物医学工程重点实验室;
【基金】：国家自然科学基金青年基金资助项目(编号:81400493) 重庆市卫生局重点项目专项资助项目(编号:2013-1-030) 2016年重庆高校创新团队建设计划资助项目(编号:CXTDG201602006)
【分类号】：R622;R-332

【相似文献】