CREG促进小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达

发布时间：2018-02-25 01:00

本文关键词： EA激活基因阻遏子巨噬细胞溶酶体　出处：《现代生物医学进展》2016年08期 　论文类型：期刊论文

【摘要】：目的:研究E1A激活基因阻遏子(Cellular repressor of E1A-stimulated genes,CREG)对小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达的影响。方法:应用loss-of-function和gain-of-function模型,Lysotracker Red染色和透射电镜观察CREG对小鼠腹腔巨噬细胞溶酶体发生的影响,并通过细胞免疫荧光染色和Western blot方法,观察CREG对小鼠腹腔巨噬细胞溶酶体组织蛋白酶表达的影响。结果:Lysotracker Red染色和透射电镜证实CREG可促进小鼠腹腔巨噬细胞溶酶体发生;细胞免疫荧光染色和Western blot方法证实CREG可促进小鼠腹腔巨噬细胞溶酶体组织蛋白酶cathepsin B及cathepsin S表达。结论:CREG可促进小鼠腹腔巨噬细胞溶酶体发生及组织蛋白酶cathepsin B及cathepsin S表达。
[Abstract]:Objective: to study the effects of Cellular repressor of E1A-stimulated genesutrid (CREGG) on lysosome formation and lysosomal cathepsin expression in murine peritoneal macrophages. Methods: the expression of lysosomal cathepsin in murine peritoneal macrophages was observed by loss-of-function and gain-of-function Red staining and transmission electron microscopy (TEM). Effects of lysosomes on peritoneal macrophages in mice, The effect of CREG on the expression of lysosomal cathepsin in murine peritoneal macrophages was observed by cell immunofluorescence staining and Western blot staining. The expression of lysosomal cathepsin B and cathepsin S in murine peritoneal macrophages was enhanced by CREG by cell immunofluorescence staining and Western blot method. Conclusion the expression of cathepsin B and cathepsin S in murine peritoneal macrophages can be enhanced by the expression of cathepsin B and cathepsin S in murine peritoneal macrophages.
【作者单位】：沈阳军区总医院全军心血管病研究所心内科;
【基金】：国家自然科学基金项目(81130072,81370243)
【分类号】：R392

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