液氮保种对田鼠巴贝虫标准株存活力的影响

发布时间：2018-02-25 02:16

本文关键词： 田鼠巴贝虫液氮保种动物保种存活力　出处：《中国人兽共患病学报》2017年07期 　论文类型：期刊论文

【摘要】：目的探索液氮保种对田鼠巴贝虫标准株存活力的影响。方法液氮保种1、3、6、9个月的田鼠巴贝虫标准株小鼠血室温复苏后感染正常BALB/c小鼠各3只(复苏1代,实验组),100μL/只;同时取3只新感染田鼠巴贝虫活体保藏小鼠为对照组。实验组染虫率达高峰时再接种正常BALB/c小鼠(复苏2代),观察田鼠巴贝虫的形态变化及增殖情况,并观察不同保存时间复苏1代及复苏2代的染虫率情况。结果液氮保种田鼠巴贝虫与活体保种的田鼠巴贝虫相比,形态变化不大,都会出现小滋养体、环状滋养体、裂殖体及未成熟和成熟裂殖子等多种形态。液氮复苏1代首次查见虫体及达到染虫率高峰的时间比动物保种延迟1~2d,但复苏2代即恢复一致。田鼠巴贝虫染虫全血经液氮保存1、3、6、9个月,仅达到高峰期时间延迟,其存活力未见明显下降。结论液氮保种对田鼠巴贝虫形态及存活力影响小,可成为一种较好的保存方式。
[Abstract]:Objective to explore the effect of liquid nitrogen conservation on the survival ability of the standard strain of Barbeiella vole. Methods the mice of the standard strain were infected with normal BALB/c mice at room temperature after blood resuscitation at room temperature for 9 months (1 generation resuscitation, 100 渭 L per mouse in the experimental group). At the same time, three newly infected mice were taken as the control group. The experimental group was inoculated with normal BALB/c mice when the infection rate reached the peak (resuscitation 2 generations), and observed the morphological changes and proliferation of Babel parasites in voles. The results showed that there was no significant change in the morphology of Bacteriella vole in liquid nitrogen conservation compared with that in living-preserved voles, and there were small trophozoites and annular trophozoites in the first generation and the second generation of resuscitation at different preservation times. In the first generation of liquid nitrogen resuscitation, the time of finding the insect and reaching the peak of the infection rate was 1 ~ 2 days later than that of the animal species conservation, but the recovery was consistent in the second generation, and the whole blood of the parasite was found in the whole blood of the first generation of water nitrogen resuscitation. It was stored in liquid nitrogen for 6 months, and was stored in liquid nitrogen for 9 months. Conclusion liquid nitrogen conservation has little effect on the morphology and survival ability of Barb 茅 a voles, and it may be a better way to preserve it.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所世界卫生组织热带病合作中心科技部国家级热带病国际联合中心卫生部寄生虫病原与媒介生物学重点实验室;复旦大学生命科学学院;
【基金】：国家科技基础条件平台专项寄生虫病和热带病科技资源中心项目上海市卫计委青年基金项目(No.20154Y0155)联合资助(No.2016)~~
【分类号】：R382.3

【相似文献】