气滞血瘀证大鼠舌部基因表达谱变化初探

发布时间：2018-02-26 03:25

本文关键词： 气滞血瘀证舌基因芯片大鼠　出处：《中国比较医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的采用基因芯片技术研究气滞血瘀证模型大鼠舌部全基因表达谱的变化以及差异基因涉及的生物过程和通路,以期为研究血瘀证相关理论和药物治疗提供科学依据。方法采用高脂饲料复合慢性不可预知刺激复制气滞血瘀动物模型,利用基因芯片技术分析正常大鼠与模型大鼠舌中全基因表达谱的变化以及所涉及通路。结果气滞血瘀证大鼠与正常大鼠比较,结果显示差异表达基因有277个,其中上调基因68个,下调基因209个。GO和Pathway分析结果显示气滞血瘀证分别与炎症、脂代谢、免疫反应等生物过程以及补体与凝血级联通路、PPAR信号通路、细胞色素P450异物代谢通路等7条通路的改变有关。结论 CYP450以及补体与凝血级联通路、PPAR信号通路中的差异基因可能涉及血瘀证的发病机制,为血瘀证以及相关药物的研究提供依据。
[Abstract]:Objective to study the changes of total gene expression profile in tongue of rats with Qi stagnation and blood stasis syndrome and the biological processes and pathways involved in differential genes by using gene chip technology. Methods the animal model of Qi stagnation and blood stasis was established by high fat diet combined with chronic unpredictable stimulation. The gene microarray technique was used to analyze the changes of the whole gene expression profile and the pathway involved in the tongue of normal and model rats. Results compared with normal rats, there were 277 differentially expressed genes in rats with Qi stagnation and blood stasis syndrome. Among them, 68 genes were up-regulated, 209 genes were down-regulated by .go and Pathway analysis showed that Qi stagnation and blood stasis syndrome were associated with inflammation, lipid metabolism, immune response, and complement and coagulation cascade pathway. Conclusion CYP450 and the differentially expressed genes in complement and coagulation cascade pathways may be involved in the pathogenesis of blood stasis syndrome. To provide the basis for the study of blood stasis syndrome and related drugs.
【作者单位】：北京中医药大学中药学院中药药理系;
【基金】：国家自然科学基金资助项目(编号:81503287,81430094) 教育部博士点基金(编号:20130013120002)
【分类号】：R228;R-332

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